Abstract
THE histochemical reaction for the localization of lactate dehydrogenase (LDH) is a multi-step reaction. The reduction of tetrazolium salts in tissue sections cannot be accomplished directly by LDH but only by means of the so-called tissue NADH-diaphorase1. Unfortunately, tissue diaphorases are not uniformly distributed in all tissues, cells or sub-cellular structures. The absence of these enzymes in certain places is therefore a limiting factor for the determination of LDH activity in tissue sections. Thus, in order to localize LDH at sites where no diaphorases are present, the use of an electron carrier seemed necessary.
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References
Pearse, A. G. E., Histochemistry, Theoretical and Applied (J. and A. Churchill, 1960).
Fahimi, H. D., and Amarasingham, C. R., Fed. Proc., 22, 195 (1963).
Fahimi, H. D., and Amarasingham, C. R., J. Cell Biol. (in the press).
van Wijhe, M., Blanchaer, M. C., and Jacyck, W. R., J. Histochem. Cytochem., 11, 505 (1963).
Fischer, R., and Benitez, L., Naturwiss. (in the press).
Benitez, L., and Fischer, R., J. Histochem. Cytochem. (in the press).
Unpublished results.
Markert, C. L., in Cytodifferentiation and Macromolecular Synthesis, edit. by Locke, M., 65 (Academic Press, New York, 1963).
Kaplan, N. O., and Ciotti, M. M., Ann. N. Y. Acad. Sci., 94, 701 (1961).
Jacob, F., and Monod, J., in Cytodifferentiation and Macromolecular Synthesis, edit. by Locke, M., 30 (Academic Press, New York, 1963).
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BENITEZ, L., FISCHER, R. Effect of Phenazine-methosulphate on the Histochemical Localization of Lactate Dehydrogenase. Nature 206, 105–106 (1965). https://doi.org/10.1038/206105a0
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DOI: https://doi.org/10.1038/206105a0
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