Abstract
RECENTLY our laboratory reported the isolation of proteolytic enzymes from a dog mast-cell tumour using DEAE-cellulose and CM-cellulose columns. An esterase which split N-acetyltyrosine ethyl ester (ATEe) and another which split p-toluene sulphonyl-l-arginine methyl ester (TAMe) were described1. Using column chromatography and eluting with a concentration gradient of phosphate buffer with a pH of 8.0, several peaks of ATEe esterase and two TAMe esterase peaks were found. All the peaks of ATEe esterase have since been found to have a similar Michaelis constant (Km) of 1.0–1.5 mM, except one which had a Km value of about 4 mM. From these observations the different peaks of ATEe esterase with similar Km values were postulated to be one enzyme.
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References
Auditore, J. V., Ende, N., and Katayama, Y., Life Sciences, 6, 375 (1963).
Kunitz, M., J. Gen. Physiol., 30, 291 (1947).
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KATAYAMA, Y., ENDE, N. Esterase Studies on Dog Mast-cell Tumours. Nature 205, 190–191 (1965). https://doi.org/10.1038/205190a0
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DOI: https://doi.org/10.1038/205190a0
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