Abstract
WE have carried out some investigations into the conformations of whole histones and histone fractions under various conditions. The experiments on repression of DNA-dependent RNA synthesis with histones1,2, native nucleohistone3, and DNA combined with histone fractions4–10 have led to a renewed interest in the hypothesis of Stedman and Stedman11 that one of the functions of histones is the regulation of genetic activity. However, the results of the various experiments on the repression of DNA-dependent RNA synthesis are not in agreement with one another, and the inhibitory capacities of the various histone fractions appear to depend on the method of preparation of the recombined nucleohistone, particularly the relative proportions of histone and DNA used for recombination, and perhaps even on the preparation of the histone fractions themselves. These apparently conflicting results indicate the need to investigate the conformations of the histone fractions, prepared by different methods, under conditions used to reconstitute the nucleohistones.
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BRADBURY, E., CRANE-ROBINSON, C., PHILLIPS, D. et al. Conformational Investigations of Histones. Nature 205, 1315–1316 (1965). https://doi.org/10.1038/2051315a0
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DOI: https://doi.org/10.1038/2051315a0
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