Abstract
FOR passive haemagglutination tests with proteins, it has been necessary to couple these antigens to erythrocytes by agents such as bis-diazotized benzidine1–3, tolylene-2,4-diisocyanate4 and tannic acid5. During the course of investigations with formalin-treated erythrocytes, we observed that bovine serum albumin (BSA) would adsorb on formalin-treated chicken erythocytes (FCE), as well as on formalin-treated sheep erythrocytes (FSE) without recourse to such coupling agents. This finding suggested a means of simplifying the procedure for coating erythrocytes with proteins for passive haemagglutination tests.
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HIRATA, A., STASHAK, P. Simplified Procedure for Passive Haemagglutination Tests with Protein Antigens. Nature 205, 1233 (1965). https://doi.org/10.1038/2051233a0
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DOI: https://doi.org/10.1038/2051233a0
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