Abstract
THE need to scan chromatographically the composition of natural oils and bacteriostatic extracts from rocks under precisely similar solvent-extraction conditions has led to the use of filter papers folded into cones. Many other fields of research require similar conditions, and it is suggested that this type of paper chromatography may have wider applications. From Fig. 1 it will be seen how simple is the equipment needed for this technique. Moreover, the apparatus enables one to obtain a solvent-saturated atmosphere very rapidly and even to work at reduced pressures. As a result of this the developing solvent rises rapidly, and at a remarkably uniform rate, so that the ingredients separate effectively into ever-narrowing zones as they ascend towards the apex of the cone (Fig. 2). These features of the technique have also proved particularly useful in concentrating antibacterial ingredients extracted from coal. The ever-narrowing front of the ascending column of ingredients results in the development of highly concentrated zones of the bacteriostatic substances, collectively known as ‘vitricin’1.
Similar content being viewed by others
Article PDF
References
Evans, W. D., Trans. Inst. Min. Metall., 65, 13 (1955).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evans, W. Conical Paper Chromatography. Nature 198, 1330 (1963). https://doi.org/10.1038/1981330a0
Issue Date:
DOI: https://doi.org/10.1038/1981330a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.