Abstract
SEMI-ISOLOGOUS (F1 hybrid) mice surviving systemic leukæmia L1210 after extensive therapy with halogenated derivatives of ‘Methotrexate’ (MTX) were shown to be immune to re-inoculation with sensitive or resistant sub-lines of leukæmia L12101–3. In other work4–6, it was observed that MTX therapy of mice bearing homografts of MTX-resistant leukæmias abrogated the immune response. However, in a system involving differences at the strong H-2 histocompatibility locus, pretreatment of mice with MTX failed to promote the growth of L12107. In the latter system, pretreatment with a number of alkylating agents (cyclophosphamide, triethylene melamine, and ‘Melphalan’) and X-irradiation resulted in marked immune suppression. In view of the foregoing results, and the observation that MTX therapy did not interfere appreciably with the ability of an inoculum of splenic tissue to render mice immune to L12105, we decided to determine what effect a dose-level of MTX capable of abrogating the immune response to tumour homograft would have on the survival of skin homografts. Also, as part of the same experiment, the effect of pro-treatment with MTX on the immune response to skin and tumour homografts was determined, since, in the present system, only minor histocompatibility differences were involved. ‘Melphalan’, a potent immune suppressor7, was used as a positive control.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Goldin, A., Humphreys, S. R., Chapman, G. O., Chirigos, M. A., and Venditti, J. M., Nature, 185, 219 (1960).
Goldin, A., and Humphreys, S. R., J. Nat. Cancer Inst., 24, 283 (1960).
Goldin, A., Humphreys, S. R., Venditti, J. M., and Mantel, N., J. Nat. Cancer Inst., 22, 811 (1959).
Potter, M., and Haas, V. H., J. Nat. Cancer Inst., 22, 801 (1959).
Humphreys, S. R., Chirigos, M. A., Milstead, K. L., Mantel, N., and Goldin, A., J. Nat. Cancer Inst., 27, 259 (1961).
Humphreys, S. R., Glynn, J. P., Chirigos, M. A., and Goldin, A., J. Nat. Cancer Inst., 28, 1053 (1962).
Humphreys, S. R., Glynn, J. P., and Goldin, A., Transplantation, 1, 65 (1963).
Friedkin, M., Crawford, E., Humphreys, S. R., and Goldin, A., Cancer Res., 22, 600 (1962).
Billingham, R. S., and Medawar, P. B., J. Exp. Biol, 28, 385 (1951).
Snell, G. D., Cancer Res., 17, 2 (1957).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GLYNN, J., BIANCO, A. & GOLDIN, A. Effect of “Methotrexate” and “Melphalan” on the Survival of Tumour and Skin Homo-grafts. Nature 198, 1003–1004 (1963). https://doi.org/10.1038/1981003b0
Issue Date:
DOI: https://doi.org/10.1038/1981003b0
This article is cited by
-
Immunodepressive activity of some radiomimetics during immunization of mice with sheep's erythrocytes
Bulletin of Experimental Biology and Medicine (1970)
-
Beeinflussung der Überlebenszeit von Hauthomotransplantaten bei Ratten durch Azathioprin
Zeitschrift für Die Gesamte Experimentelle Medizin einschließlich experimentelle Chirurgie (1969)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.