Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Resolution of Endopolygalacturonase and a Macerating Factor in a Fungal Culture Filtrate

Abstract

THE brown rot fungi, Sclerotinia fructigena Aderh. and Ruhl., and S. laxa Aderh. and Ruhl., are known to penetrate infected fruits by dissolution of the middle lamella1, and the macerating effect of culture filtrates of these fungi on disks of plant tissue has been ascribed to pectolytic enzymes2. The separate identity of ‘protopectinases’ (using the nomenclature of Demain and Phaff3) has been questioned in recent reviews3,4; but the presence of distinct fungal macerating enzymes, without characterized substrates, has been reported in a commercial poetic enzyme5 and in culture filtrates of two plant-pathogenic fungi5,6.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Valleau, W. D., J. Agric. Res., 5, 365 (1915).

    Google Scholar 

  2. Menon, K. P. V., Ann. Bot., 48, 189 (1934). Cole, J. S., Ann. Bot., N.S., 20, 15 (1956).

    Google Scholar 

  3. Demain, A. L., and Phaff, H. J., Wallerstein Labs. Commun., 20, 119 (1957).

    Google Scholar 

  4. Wood, R. K. S., Ann. Rev. Plant Physiol., 11, 299 (1960).

    Article  CAS  Google Scholar 

  5. McClendon, J. H., and Somers, G. F., Amer. J. Bot., 47, 1 (1960).

    Article  CAS  Google Scholar 

  6. Naef-Roth, S., Gaümann, E., and Albersheim, P., Phytopath. Z., 40, 283 (1961).

    Article  CAS  Google Scholar 

  7. Porath, J., and Flodin, P., Nature, 183, 1657 (1959).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Brown, W., Ann. Bot., 29, 313 (1915).

    Article  Google Scholar 

  9. Dingle, J., Reid, W. W., and Solomons, G. L., J. Sci. Food Agric., 4, 149 (1953).

    Article  CAS  Google Scholar 

  10. Peterson, E. A., and Sober, H. A., J. Amer. Chem. Soc., 78, 751 (1956).

    Article  CAS  Google Scholar 

  11. Wood, R. K. S., Ann. Bot., N.S., 19, 1 (1955).

    Article  CAS  Google Scholar 

  12. Jansen, E. F., and MacDonnell, L. R., Arch. Biochem., 8, 97 (1945).

    CAS  Google Scholar 

  13. Smith, W. K., J. Gen. Microbiol., 18, 33 (1958).

    Article  CAS  PubMed  Google Scholar 

  14. Tribe, H. T., Ann. Bot., N.S., 19, 351 (1955).

    Article  Google Scholar 

  15. Brown, W., Ann. App. Biol., 43, 325 (1955).

    Article  Google Scholar 

  16. Warburg, O., and Christian, W., Biochem. Z., 310, 384 (1941).

    Google Scholar 

  17. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

BYRDE, R., FIELDING, A. Resolution of Endopolygalacturonase and a Macerating Factor in a Fungal Culture Filtrate. Nature 196, 1227–1228 (1962). https://doi.org/10.1038/1961227a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/1961227a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing