Abstract
EXCEPT when isotopes have been used, the investigation of the metabolism of the sugar phosphates and related compounds in animal and plant tissues has long been restricted by a lack of specific methods of determination1. Recently, however, specific enzymatic techniques have been developed for the estimation of both sugar phosphates and glycolytic intermediates in extracts from animal and plant tissues2–7. The enzymatic methods present greater difficulties with plant than with animal extracts, partly because the contents of phosphate compounds are smaller, and partly because the presence of pigments and inhibitors interferes with the assays. But successful results have now been obtained with extracts of potato tubers, strawberry leaves and fruits of banana, tomato and apple.
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Marré, E., Ann. Rev. Plant Physiol., 12, 195 (1961).
Slater, E. C., Biochem. J., 53, 157 (1953).
Aisenburg, A. C., J. Biol. Chem., 234, 441 (1959).
Morgan, H. E., Randle, P. J., and Regen, D. M., Biochem. J., 73, 573 (1959).
Newsholme, E. A., and Randle, P. J., Biochem. J., 80, 655 (1961).
Fawaz, E. A., and Fawaz, G., Biochem. J., 83, 438 (1962).
Rowan, K. S., J. Exp. Bot., 9, 436 (1958).
Isherwood, F. A., and Barrett, R. C. (private communication, 1960).
Seraydarian, K., Mommaerts, W. F. H. M., and Walker, A., J. Biol. Chem., 235, 2191 (1960).
Barker, J., and Solomos, T., Nature, 196, 189 (1962).
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BARKER, J., ISHERWOOD, F., JAKES, R. et al. Determination of Certain Phosphate Compounds in Plant Extracts. Nature 196, 1115 (1962). https://doi.org/10.1038/1961115a0
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DOI: https://doi.org/10.1038/1961115a0
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