Abstract
BY the use of DEAE-cellulose chromatography, we have endeavoured to obtain in their pure form various factors developed by adenoviruses during their propagation in tissue culture cells1–4. HeLa or human amnion cells were inoculated with adenoviruses of types 1, 2, 3, 5, 7a, 9, 10, 11 and 15. On the 3rd–5th day the culture fluids were collected and were dialysed against 0.005 M borate buffer solution, pH 8.10. The dialysed fluid was then introduced into a DEAE-cellulose column (1 cm. diam., 10 cm. high) which was previously balanced by 0.005 M borate buffer solution, pH 8.10. Fractionation was carried out by adding 0.005 M borate buffer solution, pH. 8.10, containing sodium chloride, to the column in linearly increasing concentrations of 0–0.8 M. 5-ml. fractions were collected and analysed with the view of detecting hæmagglutinins (by the reaction of hæmagglutination with the erythrocytes of rats and monkeys), also an early cytopathogenic factor5,6, the complement-fixing antigen and an infectious virus.
Similar content being viewed by others
Article PDF
References
Sober, H. A., and Peterson, E. A., J. Amer. Chem. Soc., 78, 751 (1956).
Klemperer, H. G., and Pereira, H. G., Virology, 9, 536 (1959).
Philipson, L., Virology, 10, 459 (1960).
Haruna, J., Yaoi, H., Kono, R., and Watanabe, J., Virology, 13, 267 (1961).
Pereira, H. G., Virology, 9, 601 (1958).
Everett, S. F., and Gingberg, H. S., Virology, 9, 770 (1958).
Salganik, G. I., Scherbatich, V. P., and Protas, L. K., Physiology and Genetics of Viruses (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ZHDANOV, V., MEKLER, L. Correlation between the Surface Structure of Viruses and Latency. Nature 195, 924 (1962). https://doi.org/10.1038/195924a0
Issue Date:
DOI: https://doi.org/10.1038/195924a0
This article is cited by
-
Separation of adenovirus type 4 components
Archiv f�r die gesamte Virusforschung (1967)
-
DEAE-Chromatography of Adenoviruses of Rosen's Group II
Nature (1965)
-
Untersuchungen �ber Adenovirus-H�magglutinine der Gruppe I nach Rosen
Archiv f�r die gesamte Virusforschung (1965)
-
Eigenschaften der Adenovirus-Hämagglutinine
Zeitschrift für Hygiene und Infektionskrankheiten (1963)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.