Abstract
CONTINUING our work on the isotope effects of deuterium on living systems1–3 the effects of 99.6 per cent deuterium oxide on the growth of two species of yeast have been investigated in this laboratory. Attempts to cultivate a strain of Torulopsis utilis (ATCC No. 8205) in a medium composed of inorganic salts and glucose4 in deuterium oxide were not successful although a medium of identical composition in ordinary water supported good growth of this organism. Addition to the D2O-medium of small amounts of intracellular components of algae grown in heavy water3 promoted growth of this yeast. (Reference in the text to H2O- or D2O-medium implies nutrient solutions composed of inorganic salts, ammonium sulphate as the nitrogen source, and glucose (after Wickerham4) in distilled water or 99.6 per cent deuterium oxide.) Fig. 1 shows a growth curve of T. utilis in a D2O-medium supplemented with a deuterated extract of algae.
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References
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MOHAN, V., CRESPI, H. & KATZ, J. Nutritional Requirements for the Cultivation of Fully Deuterated Yeasts Torulopsis utilis and Saccharomyces cerevisiae. Nature 193, 189–190 (1962). https://doi.org/10.1038/193189a0
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DOI: https://doi.org/10.1038/193189a0
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