Abstract
PREVIOUS reports from this Laboratory1–3 have shown that human growth hormone (HGH) and sheep interstitial cell stimulating hormone (ICSH) produce, in rabbits, precipitin antibodies that are specific to the respective hormones. Immunochemical techniques have been used to characterize these hormones as well as to perform quantitative determinations of small quantities of the hormone. Hæmagglutination inhibition has also been used to study and detect HGH in human serum4. The purpose of the present investigation was to study HGH and sheep ICSH by another immunochemical reaction, namely, complement fixation. It may be noted that the complement fixation method is capable of determining quantitatively as little as 0.01 µgm. of the hormone and is an objective procedure which is more reproducible than the hæmagglutination technique.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hayashida, T., and Li, C. H., Science, 128, 1276 (1958).
Li, C. H., Moudgal, N. R., and Papkoff, H., J. Biol. Chem., 235, 1038 (1960).
Moudgal, N. R., and Li, C. H., Arch. Biochem. and Biophys. (in the press).
Read, C. H., and Bryan, G. T., in Ciba Foundation Colloquia on Endocrinology, 13, 68 (1960).
Kabat, E. A., and Mayer, M. M., Experimental Immunochemistry, 97 (Charles Thomas, Springfield, Illinois, 1948).
Greenspan, F. S., Li, C. H., Simpson, M. E., and Evans, H. M., Endocrinol., 45, 455 (1949).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TRENKLE, A., MOUDGAL, N., SADRI, K. et al. Complement-fixing Antibodies to Human Growth Hormone and Sheep Interstitial Cell Stimulating Hormone. Nature 192, 260–261 (1961). https://doi.org/10.1038/192260a0
Issue Date:
DOI: https://doi.org/10.1038/192260a0
This article is cited by
-
Flocculation reaction in a system human growth hormone ? Antihormonal horse serum
Bulletin of Experimental Biology and Medicine (1968)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.