Abstract
A PREVIOUS report from this laboratory1 described the kinetics of uptake of streptomycin labelled with carbon-14 by Escherichia coli W growing in mineral-citrate-glucose medium A2. In the procedure used, the cells were separated from the medium on a membrane filter and washed with distilled water before determination of radioactivity. We now find that the uptake had been markedly enhanced by a step not reported previously: dilution of each sample with several volumes of distilled water immediately before filtration. (This predilution in water was considered trivial because the subsequent washing with water removed little of the radioactivity on the filter, although almost all of it could be removed by washing with medium A.) When this predilution step was omitted the radioactivity retained on the filter was lower by 50 to 80 per cent (Fig. 1), but there was still, as previously reported, an immediate ‘primary’ uptake and, after a lag, a gradually increasing ‘secondary’ uptake. (Szybalski and Mashima3 have reported kinetics of streptomycin uptake which are difficult to compare with ours since their first sample was not taken until 1 hr. had elapsed and they measured the radioactivity retained after washing with non-radioactive streptomycin.)
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Anand, N., Davis, B. D., and Armitage, A. K., Nature, 185, 23 (1960).
Davis, B. D., and Mingioli, E. S., J. Bacteriol., 60, 17 (1950).
Szybalski, W., and Mashima, S., Biochem. Biophys. Res. Comm., 1, 249 (1959).
Green, S. R., and Waksman, S. A., Proc. Soc. Exp. Biol. and Med., 67, 281 (1948).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PLOTZ, P., DUBIN, D. & DAVIS, B. Influence of Salts on the Uptake of Streptomycin by Escherichia coli. Nature 191, 1324–1325 (1961). https://doi.org/10.1038/1911324a0
Issue Date:
DOI: https://doi.org/10.1038/1911324a0
This article is cited by
-
Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters
Scientific Reports (2015)
-
Streptomycin potency is dependent on MscL channel expression
Nature Communications (2014)
-
Dihydrostreptomycin accumulation in E. coli
Nature (1974)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.