Abstract
PREPARATIONS of heparin have been shown to be heterogeneous by a variety of techniques1,2, but no method is available for the fractionation of bulk quantities of heparin or of other sulphomucopolysaccharides. These compounds carry a strong negative charge over a wide range of pH, and, therefore, it seemed possible that they could be purified on basic resins. Five anion-exchange resins (‘Dowex–1’, ‘Dowex–2’, ‘Dowex–3’, ‘Amberlite CG–45’, and ‘Duolite A–4’), each buffered at pH 6.0, readily adsorbed heparin, which was easily eluted with strong salt solutions, but the eluates differed from the original material in having greater absorption at 400 mµ than at 535 mµ in the carbazole reaction3. However, ‘Ecteola’4,5 (exchange capacity = 0.3 m.equiv./gm.) resolved a commercial preparation of bovine heparin (obtained from Nutritional Biochemicals Corp.) into four major and one minor carbazole-positive fractions (Fig. 1), each with maximum absorption at 535 mµ, and all showing metachromasia6. 90 per cent of the original material was recovered. Heparin-35SO4, extracted from a mast-cell tumour of the mouse7, gave only one peak on chromatography on ‘Ecteola’ (Fig. 2), and only 50 per cent of the material was eluted.
Similar content being viewed by others
Article PDF
References
Foster, A. B., and Huggard, A. J., “Advances in Carbohydrate Chemistry”, 10, 335, edit. by Wolfrom, M. L. (Academic Press, New York, 1955).
Jaques, L. B., Bell, H. J., and Cho, M. H., in First International Conference on Thrombosis and Embolism, 281 (Benno Schwabe and Co., Basle, 1955).
Dische, Z., in “Methods of Biochemical Analysis”, 2, 313, edit. by Glick, D. (Interscience Publishers, New York, 1955).
Peterson, E. A., and Sober, H. A., J. Amer. Chem. Soc., 78, 751 (1956).
Bendich, A., Pahl, H. B., Korngold, G. C., Rosenkranz, H. S., and Fresco, J. R., J. Amer. Chem. Soc., 80, 3949 (1958).
Roberts, M., Green, J. P., and Day, S. M., Fed. Proc., 18, 438 (1959).
Green, J. P., and Day, M., Biochem. Pharm. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GREEN, J. Fractionation of Heparin on an Anion Exchanger. Nature 186, 472 (1960). https://doi.org/10.1038/186472a0
Issue Date:
DOI: https://doi.org/10.1038/186472a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.