Abstract
THE traditional method of preparing kidney for microdissection is by maceration of formalin-fixed tissue with concentrated hydrochloric acid1. This method gives an, excellent preparation of loosened nephrons and collecting tubules that can be disentangled and studied by microdissection. However, it suffers from the disadvantage that the acid treatment changes the nuclei so that they cannot be demonstrated in dissected preparations by the usual staining methods. A method of maceration that would allow nuclear staining after dissection would considerably widen the scope of the technique.
Similar content being viewed by others
Article PDF
References
Oliver, J., MacDowell, M., and Tracy, A., J. Clin. Invest., 30, 1307 (1951).
Darmady, E. M., and Stranack, F., Brit. Med. Bull., 13, 21 (1957).
Neuman, R. E., and Tytell, A. A., Proc. Soc. Exp. Biol. N.Y., 73, 409 (1950).
Robb-Smith, A. H. T., “Nature and Structure of Collagen”, edit. by Randall, J. T., 21 (Butterworths, London, 1953).
Keech, M. K., J. Path. Bact., 77, 351 (1959).
Clarke, J. L., Phil. Trans. Roy. Soc., B, 141, 607 (1851).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HAYWARD, N. Collagenase Maceration of Kidney for Microdissection. Nature 186, 403 (1960). https://doi.org/10.1038/186403a0
Issue Date:
DOI: https://doi.org/10.1038/186403a0
This article is cited by
-
Renin release from isolated afferent arterioles
Kidney International (1985)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.