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Immunochemical Observations on Antisera to Purified Human Growth Hormone

Abstract

RECENTLY, Hayashida and Li1 and Read2 have reported that purified preparations of human growth hormone were antigenic when injected with adjuvants into rabbits. Serum antibodies were demonstrated by the precipitin ring test1 and by a modification2 of the tanned cell hæmagglutination method of Boyden3. It was also shown that the rabbit antiserum inhibited the biological activity of human growth hormone1. Read2 has employed rabbit antiserum to measure the concentration of growth hormone in human serum by the hæmagglutination–inhibition reaction3,4. It seemed essential in assessing the specificity of this method to investigate the purity of the antigen and the specificity of the antisera. In this report immunochemical evidence is presented which confirms that human growth hormone is a potent antigen. In addition, results were obtained which indicate that the Raben preparation of human growth hormone5 is a highly purified antigen.

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GRUMBACH, M., KAPLAN, S. & SOLOMON, S. Immunochemical Observations on Antisera to Purified Human Growth Hormone. Nature 185, 170–172 (1960). https://doi.org/10.1038/185170a0

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