Abstract
UNTIL recently, genetical studies with Salmonella typhimurium have been carried out mainly by the transduction method. In an effort to facilitate such studies, we attempted to develop a means of applying conjugation techniques also, using S. typhimurium and Escherichia coli, since Baron and his collaborators1 had been able to produce hybrids between these two bacteria. We have been successful in experiments with a Salmonella culture carrying a mutator gene (mut) and a culture of E. coli strain K-12 carrying the factor for high frequency of recombination (Hfr). The mutator strain was isolated by Miyake2 from strain LT-7. Its main characteristic is a 10- to 100-fold increase in spontaneous mutability.
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Baron, L. S., Carey, W. F., and Spilman, W. M., Abst. Comm. at Paper Sessions, Seventh Int. Congr. Microbiol. (1958).
Demerec, M., Lahr, E. L., Miyake, T., Goldman, I., Balbinder, E., Banic, S., Hashimoto, K., Glanville, E. V., and Gross, J. D., Carnegie Inst. Wash. Year Book, 57, 390 (1958).
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MIYAKE, T., DEMEREC, M. Salmonella–Escherichia Hybrids. Nature 183, 1586 (1959). https://doi.org/10.1038/1831586a0
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DOI: https://doi.org/10.1038/1831586a0
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