Abstract
A SIMPLE and rapid paper chromatographic method was required for separating mixtures of isomers of benzene hexachloride. The paper chromatographic method of Moynihan and O'Colla1, further developed by O'Colla2 and Mitchell3, gives a satisfactory separation, but the acetic anhydride, used as the stationary phase, is an unpleasant reagent to handle. The use of ‘Vaseline’-impregnated strips has been previously described for the separation of DDT derivatives4, and there was some evidence that separation of isomers of benzene hexachloride could be achieved by the application of a similar technique5. The method has been adapted as follows.
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References
Moynihan, P., and O'Colla, P., Chem. and Indust., 21, 407 (1951).
O'Colla, P., J. Sci. Food Agric., 3, 130 (1952).
Mitchell, L. C., J. Assoc. Off. Agric. Chem., 35, 920 (1952).
Winteringham, F. P. W., Harrison, A., and Bridges, R. G., Nature, 166, 999 (1950).
Winteringham, F. P. W., Harrison, A., and Bridges, R. G., Analyst, 77, 19 (1952).
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BRIDGES, R., HARRISON, A. & WINTERINGHAM, F. Separation of the Isomers of Benzene Hexachloride by Reversed-Phase Paper-Partition Chromatography. Nature 177, 86 (1956). https://doi.org/10.1038/177086a0
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DOI: https://doi.org/10.1038/177086a0
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