Abstract
Trichomonas hominis, like most other intestinal parasites1, is usually cultured in vitro in a Dobell–Laidlaw biphasic medium (horse serum, rice starch and Ringer's solution to which is added 15 per cent liquid horse serum)2. Growth of T. hominis in that medium occurs only when associated with bacteria. At 25° C. the equilibrium between bacteria and protozoa is optimum, and after forty days of incubation, Trichomonas are still numerous and lively. In such conditions, the maximum protozoal concentration attained is about 200,000 cells/c.c.
Similar content being viewed by others
Article PDF
References
Lamy, L., and Lambault, E., Bull. Soc. Path. Exotique, 45, 186 (1952).
Dobell, C., and Laidlaw, P. P., Parasit., 18, 283 (1926). Brumpt, E., Bull. Méd., 40, 105 (1926).
Sorel, C., Presse Méd., 62, 602 (1954).
McEntegart, M. G., J. Hyg., 52, 545 (1954).
Shaffer, J. G., and Biegeleisen, J. Z., Amer. J. Hyg., 53, 139 (1951).
Johnson, G., and Trussell, R. E., Proc. Soc. Exp. Biol. Med., 54, 245 (1943).
McEntegart, M. G., J. Clin. Path., 5, 275 (1952).
Feinberg, J. G., Nature, 171, 1165 (1953).
Williams, L. F., and Plastridge, V. N., J. Bact., 51, 127 (1946).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DE CARNERI, I. Trichomonas hominis cultured without Bacteria. Nature 176, 605 (1955). https://doi.org/10.1038/176605a0
Issue Date:
DOI: https://doi.org/10.1038/176605a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.