Abstract
BULL semen for artificial insemination is usually stored at 4° C. in media composed of equal volumes of either phosphate buffer (pH 7.4) or citrate buffer (pH 6.8) and fresh egg-yolk1. Under these conditions the motility and fertilizing capacity of spermatozoa decrease rapidly with time, and in practice samples are not used for insemination for more than three days after collection. Semen can be successfully stored for much longer periods at − 79° C.2, but this technique has disadvantages for everyday use.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Rothschild, Lord, and Barnes, H., Nature, 173, 636 (1954). Campbell, R. C., and Edwards, J., Nature, 173, 637 (1954).
Polge, C., and Rowson, L. E. A., Rep. 2nd Int. Congr. Physiol. Path. Anim. Reproduction and Artificial Insemination, 3, 90 (1952).
Bishop, M. W. H., and Salisbury, G. w., Amer. J. Physiol. (in the Press).
Sørensen, S. P. L., Ergebn. Physiol., 12, 393 (1912).
Tyler, A., Biol. Bull., 104, 224 (1953). Lorenz, F. W., and Tyler, A., Proc. Soc. Exp. Biol. Med., 78, 57 (1951). Tyler, A., and Tanabe, T. Y., Proc. Soc. Exp. Biol. Med., 81, 367 (1952).
Tyler, A., and Rothschild, Lord, Proc. Soc. Exp. Biol. Med., 76, 52 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ROY, A., BISHOP, M. Effect of Glycine on the Survival of Bull Spermatozoa in vitro . Nature 174, 746–747 (1954). https://doi.org/10.1038/174746a0
Issue Date:
DOI: https://doi.org/10.1038/174746a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
