Abstract
DURING an electron microscopic investigation of Bacterium coli, a variant form of this bacterium was observed with several interesting features. The accompanying electron micrographs 1(a) and 1(b) show the normal types of this bacterium usually obtained. In both cases bacteria were grown on Morton–Engley's enriched agar medium1 and incubated for 2½ hr. at 37° C. Bacteria represented in Fig. 1(a) were fixed by exposure to vapour of 2 per cent osmic acid, whereas those of Fig. 1(b) were fixed in 5 per cent formalin. Those fixed in formalin always showed a greater shrinkage of protoplasm from the cell wall, compared to those fixed in osmic acid vapour.
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References
Morton, H. E., and Engley, F. B., J. Bact., 49, 245 (1945).
Hillier, J., Mudd, S., and Smith, A. G., J. Bact., 57, 319 (1949).
Vaureka, A., Lancet, i, 27 (1951).
Eisenstark, A., and Clark, G. L., Science, 105, 553 (1947).
Newcombe, H. B., Nature, 164, 150 (1949).
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DE, M., GUHA, A. & DAS GUPTA, N. A Variant of Bacterium coli. Nature 171, 879–880 (1953). https://doi.org/10.1038/171879a0
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DOI: https://doi.org/10.1038/171879a0
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