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Stem Cell Procurement

Hourly monitoring of circulating CD34+ cells to optimize timing of autologous apheresis in pediatric patients

Bone Marrow Transplantation volume 36, pages 481–489 (2005)Cite this article

Summary:

In order to increase the CD34+ cell yield in children undergoing autologous stem cell transplantation, the optimum time of apheresis after G-CSF administration has still to be found. We prospectively studied the mobilization of CD34+ cells and white blood cells in the peripheral blood (PB) of 20 pediatric patients before leukapheresis. The monitoring schedule covered 12 h, with blood samples taken before and at 2, 4, 5, 6, 7, 8, 10 and 12 h after G-CSF administration when 10 CD34+ cells/μl were reached. CD34+ cells were measured by flow cytometric analysis both in the single- and dual-platform setting. Two different patterns of mobilization (POM) emerged: 12 patients showed an increase in CD34+ cells in PB during the first 4 h after G-CSF (POM I), while eight patients had an initial decrease of CD34+ cells. However, all patients together showed a significant increase of CD34+ cells about 10 h after G-CSF administration. Further studies with more patients, using an enhanced monitoring schedule will be required to refine the results.

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Acknowledgements

We thank all patients and their parents for their compliance and support. Further thanks go to ‘Verein Hilfe für krebskranke Kinder Frankfurt e.V.’ for supporting this study.

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Authors and Affiliations

  1. Pediatric Hematology and Oncology of Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, Frankfurt am Main, Germany

    K Bochennek, C Andreas, C Margraf, H Stahlkamp, S Zimmermann, R Esser, D Schwabe, T Klingebiel, H-P Grüttner & U Koehl

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  1. K Bochennek
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  2. C Andreas
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Correspondence to U Koehl.

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Bochennek, K., Andreas, C., Margraf, C. et al. Hourly monitoring of circulating CD34+ cells to optimize timing of autologous apheresis in pediatric patients. Bone Marrow Transplant 36, 481–489 (2005). https://doi.org/10.1038/sj.bmt.1705098

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