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Pre-Clinical Studies

Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

Bone Marrow Transplantation volume 36, pages 355–365 (2005)Cite this article

Summary:

Human mesenchymal stem cells (hMSCs) are excellent candidates for ex vivo gene transfer and cell therapy in various systems. However, hMSCs are mortal somatic cells, and thus invariably enter an irreversible growth arrest after a finite number of cell divisions in culture. It has been proposed that this is due to telomere shortening. In this study, pGRN145 plasmid containing human telomerase reverse transcriptase (hTRT) was introduced into fetal dermis-derived hMSCs. Single-cell clones positive for telomerase activity and hTRT mRNA were selected and expanded. Single-cell-derived hTRT+ cells could be expanded rapidly in vitro and passaged up to 70 doublings without showing senescence. FACScan flow cytometer showed that hTRT+ cells were positive for CD29, CD44, CD105, and CD166, while CD31, CD45, CD34, vWF, and HLA-DR were negative. Under suitable conditions, hTRT+ cells have the ability of multiple lineage differentiation, including bone, fat, and nerve. Furthermore, transplantation of hTRT+ cells could protect NOD/SCID mice from lethal irradiation. Thus, these cells may be an ideal cell source for promoting hematopoietic recovery after radiotherapy.

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Acknowledgements

We thank Li Ma, Shaoguang Yang, and Mingxia Shi for technical help. This study was supported by grants from the ‘863 Projects’ of Ministry of Science and Technology of PR China (No. 2002AA205061); from China Medical Board of New York Inc.: Stem Cell Biology, Engineering (Grant #01-748); from National Natural Science Foundation of China (No. 30070284); from National Key Project for Basic Research of China (No. 001CB5099); from Peking Ministry of Science and Technology (No. 2002-489). RCH Zhao is a Cheung Kong Scholar in PR China.

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Authors and Affiliations

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, People's Republic of China

    Z Zhao, X Jiang & R C Zhao

  2. Institute of Basic Medical Sciences and School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China

    L Liao, Y Cao & R C Zhao

  3. Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China

    L Liao, Y Cao & R C Zhao

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  1. Z Zhao
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Correspondence to R C Zhao.

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Zhao, Z., Liao, L., Cao, Y. et al. Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo. Bone Marrow Transplant 36, 355–365 (2005). https://doi.org/10.1038/sj.bmt.1705062

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