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Donor DNA in a renal cell carcinoma metastasis from a bone marrow transplant recipient

Bone Marrow Transplantation volume 34, pages 183–186 (2004)Cite this article

Individuals receiving either allogeneic bone marrow transplants,1 or organ transplants,2 are at an increased risk of developing de novo malignancies. Radiation and immunosuppression are both risk factors. As unfortunate as this is, it could allow for detection of tumor cell–hematopoietic cell hybrids in human cancer. In animal models, tumor hybrids were documented through the use of heterologous genetic markers for the tumor and host genotypes, in some cases revealing hybridization with hematopoietic cells.3, 4 In noncancer systems, heterologous markers were used to demonstrate fusion of bone marrow-derived stem cells with tissue cells.5, 6, 7

Tumor cell DNA was amplified using the 176 bp primers, aliquots were subjected to KpnI digestion, and the mixtures were run on agarose gels. Results for tumor samples T2 and T6 are in Figure 2b. Without KpnI (−), a single 176 bp band was seen, whereas with KpnI (+) two bands of 176 and 150 bp were seen, consistent with being A and O allele fragments. Similarly, amplification of tumor samples T5 and T6 with the 193 bp primers and KpnI digestion yielded bands of 193 and 174 bp, as predicted for A and O alleles (Figure 2c). KpnI digestion of tumor sample T19 produced a 193 bp A allele fragment, but no O allele fragment (Figure 2c).

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Authors and Affiliations

  1. Department of Dermatology and Yale Cancer Center, Yale School of Medicine, New Haven, CT, USA

    A Chakraborty, R Lazova & J Pawelek

  2. Department of Pediatrics, Cincinnati Children's Hospital, Cincinnati, OH, USA

    S Davies

  3. Department of Genetics and Pathology, Uppsala University School of Medicine, Uppsala, Sweden

    H Bäckvall & F Ponten

  4. Departments of Therapeutic Radiology and Genetics, Yale School of Medicine, New Haven, CT, USA

    D Brash

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  1. A Chakraborty
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  2. R Lazova
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  7. J Pawelek
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Chakraborty, A., Lazova, R., Davies, S. et al. Donor DNA in a renal cell carcinoma metastasis from a bone marrow transplant recipient. Bone Marrow Transplant 34, 183–186 (2004). https://doi.org/10.1038/sj.bmt.1704547

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