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CD34+ Numbers

Ideal or actual body weight to calculate CD34+ cell doses for autologous hematopoietic stem cell transplantation?

Bone Marrow Transplantation volume 31, pages 861–864 (2003)Cite this article

Summary:

The number of CD34+ cells infused influences the speed of hematologic recovery post-transplant. There are limited data on whether ideal (IBW) or actual (ABW) body weight should be used to calculate CD34+ cell dose. We compared the correlation between recovery to 0.5 × 109/l neutrophils and the CD34+ cell dose based upon ABW as well as IBW in 87 patients autografted for cancer. ABW was ⩾25% over IBW in 43% of patients. The median number of CD34+ cells administered was 3.6 × 106/kg ABW and 4.2 × 106/kg IBW. The time to neutrophil recovery was 8–15 days (median 10). There was a stronger inverse correlation between CD34+ cell dose/IBW and neutrophil recovery (r2=0.308; P<0.0001) than between CD34+ cell dose/ABW and neutrophil recovery (r2=0.267; P<0.0001). The median time to neutrophil recovery was comparable for those receiving ⩾2 × 106/kg CD34+ cells/kg IBW as well as ABW (10 days) and those receiving ⩾2 × 106/kg CD34+ cells/kg IBW but <2/kg ABW (10 days), but was significantly slower for those receiving <2 × 106/kg CD34+ cells/kg IBW (12 days). These data show that the CD34+ cell dose based on IBW is a better predictor of neutrophil recovery after autotransplantation.

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References

  1. Mehta J, Powles R, Horton C et al. Factors affecting engraftment and hematopoietic recovery after unpurged autografting in acute leukemia. Bone Marrow Transplant 1996; 18: 319–324.

    CAS  PubMed  Google Scholar 

  2. Bensinger WI, Longin K, Appelbaum F et al. Peripheral blood stem cells (PBSCs) collected after recombinant granulocyte colony stimulating factor (rhG-CSF): an analysis of factors correlating with the tempo of engraftment after transplantation. Br J Haematol 1994; 87: 825–831.

    Article  CAS  PubMed  Google Scholar 

  3. Weaver CH, Hazelton B, Birch R et al. An analysis of engraftment kinetics as a function of the CD34 content of peripheral blood progenitor cell collections in 692 patients after the administration of myeloablative chemotherapy. Blood 1995; 86: 3961–3969.

    CAS  PubMed  Google Scholar 

  4. Ketterer N, Salles G, Raba M et al. High CD34(+) cell counts decrease hematologic toxicity of autologous peripheral blood progenitor cell transplantation. Blood 1998; 91: 3148–3155.

    CAS  PubMed  Google Scholar 

  5. Shpall EJ, Champlin R, Glaspy JA . Effect of CD34+ peripheral blood progenitor cell dose on hematopoietic recovery. Biol Blood Marrow Transplant 1998; 4: 84–92.

    Article  CAS  PubMed  Google Scholar 

  6. Waples JM, Moreb JS, Sugrue M et al. Comparison of autologous peripheral blood stem cell dosing by ideal vs actual body weight. Bone Marrow Transplant 1999; 23: 867–873.

    Article  CAS  PubMed  Google Scholar 

  7. Mehta J, Powles R, Treleaven J et al. Long-term follow-up of patients undergoing allogeneic bone marrow transplantation for acute myeloid leukemia in first complete remission after cyclophosphamide-total body irradiation and cyclosporine. Bone Marrow Transplant 1996; 18: 741–746.

    CAS  PubMed  Google Scholar 

  8. Mehta J, Powles R, Treleaven J et al. Number of nucleated cells infused during allogeneic and autologous bone marrow transplantation: an important modifiable factor influencing outcome. Blood 1997; 90: 3808–3810.

    CAS  PubMed  Google Scholar 

  9. Singhal S, Powles R, Kulkarni S et al. Comparison of marrow and blood cell yields from the same donors in a double-blind, randomized study of allogeneic marrow vs blood stem cell transplantation. Bone Marrow Transplant 2000; 25: 501–505.

    Article  CAS  PubMed  Google Scholar 

  10. Mehta J, Oyama Y, Winter J et al. CD34+ cell collection efficiency does not correlate with the pre-leukapheresis hematocrit. Bone Marrow Transplant 2001; 28: 597–601.

    Article  CAS  PubMed  Google Scholar 

  11. Mehta J, Singhal S, Gordon L et al. Cobe Spectra is superior to Fenwal CS 3000 Plus for collection of hematopoietic stem cells. Bone Marrow Transplant 2002; 29: 563–567.

    Article  CAS  PubMed  Google Scholar 

  12. Sutherland DR, Anderson L, Keeney M et al. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother 1996; 5: 213–226.

    Article  CAS  PubMed  Google Scholar 

  13. Devine DJ . Gentamicin therapy. Drug Intell Clin Pharm 1974; 8: 650–655.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported in part by the Auxiliary Board of the Northwestern Memorial Hospital.

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Authors and Affiliations

  1. Division of Hematology/Oncology, Hematopoietic Stem Cell Transplant Program, The Feinberg School of Medicine, Northwestern University, The Robert H Lurie Comprehensive Cancer Center of Northwestern University, Chicago, IL, USA

    M Y Ali, Y Oyama, J Monreal, J N Winter, M S Tallman, S F Williams, S Singhal, L I Gordon & J Mehta

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  1. M Y Ali
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  2. Y Oyama
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  3. J Monreal
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  4. J N Winter
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  5. M S Tallman
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  6. S F Williams
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  7. S Singhal
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  8. L I Gordon
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  9. J Mehta
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The first two authors contributed equally to this work.

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Ali, M., Oyama, Y., Monreal, J. et al. Ideal or actual body weight to calculate CD34+ cell doses for autologous hematopoietic stem cell transplantation?. Bone Marrow Transplant 31, 861–864 (2003). https://doi.org/10.1038/sj.bmt.1703955

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  • DOI: https://doi.org/10.1038/sj.bmt.1703955

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