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Technique for PBSC harvesting in children of weight under 10 kg

Bone Marrow Transplantation volume 29, pages 57–61 (2002)Cite this article

Abstract

Peripheral blood stem cell (PBSC) harvesting in the smallest children (weight <10 kg) using separators is complicated by specific problems. The volume of the separation set exceeds 25% of the total blood volume and the vascular access is generally not sufficient. Therefore, a simple manual technique for PBSC harvesting was developed. Three children (6–9 kg), with newly diagnosed tumours were scheduled to be treated with three to six sequential courses of high-dose chemotherapy, each followed by PBSC support. PBSC harvests were started after mobilization using cyclophosphamide and G-CSF when the peripheral blood CD34+ cell count exceeded 50/μl. About 50 ml of blood was drawn from a venous catheter, injected into a transfer bag containing ACD-A, and centrifuged. The buffy coat obtained was pooled in a collection bag, remaining plasma and erythrocytes were immediately reinfused and a subsequent cycle started. From three to 13 cycles were performed in 1–3 days and 18.0–32.2 × 106 CD34+cells/kg were collected. We did not detect any bacterial contamination or any notable complications. Fifteen PBSC reinfusions have been performed to date, each with rapid engraftment taking between 7 and 13 days. Patients are in very good PR (18 months from diagnosis) or in CR (6 and 8 months). We can conclude that this procedure is feasible and safe.

Bone Marrow Transplantation (2002) 29, 57–61. doi:10.1038/sj.bmt.1703334

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References

  1. Auquier P, Macquart-Moulin G, Moatti JP et al. Comparison of anxiety, pain and discomfort in two procedures of hematopoietic stem cell collection: leukacytapheresis and bone marrow harvest Bone Marrow Transplant 1995 16: 541 547

    CAS  PubMed  Google Scholar 

  2. To LB, Haylock DN, Simmons PJ et al. The biology and clinical use of blood stem cells Blood 1997 89: 2233 2258

    CAS  PubMed  Google Scholar 

  3. Demeocq F, Kanold J, Chassagne J et al. Successful blood stem cell collection and transplant in children weighing less than 25 kg Bone Marrow Transplant 1994 13: 43 50

    CAS  PubMed  Google Scholar 

  4. Gorlin JB, Humphreys D, Kent P et al. Pediatric large volume peripheral blood progenitor cell collections from patients under 25kg: a primer J Clin Apher 1996 11: 195 203

    Article  CAS  PubMed  Google Scholar 

  5. Nussbaumer W, Schönitzer D, Trieb T et al. Peripheral blood stem cell (PBSC) collection in extremely low-weight infants Bone Marrow Transplant 1996 18: 15 17

    CAS  PubMed  Google Scholar 

  6. Pahys J, Fisher V, Carneval M et al. Successful large volume leukapheresis on a small infant allogeneic donor Bone Marrow Transplant 2000 26: 339 341

    Article  CAS  PubMed  Google Scholar 

  7. Kořístek Z, Štěrba J, Mayer J et al. PBSC collection using COBE Spectra in small children – single center experience Bone Marrow Transplant 2001 27: (Suppl. 1) S302

    Google Scholar 

  8. Gianni AM . Where do we stand with respect to the use of peripheral blood progenitor cells? Ann Oncol 1994 5: 781 784

    Article  CAS  PubMed  Google Scholar 

  9. Kroger N, Zeller W, Hassan HT et al. Stem cell mobilization with G-CSF alone in breast cancer patients: higher progenitor cell yield by delivering divided doses (2 × 5 μg/kg) compared to a single dose (1 × 10 μg/kg) Bone Marrow Transplant 1999 23: 125 129

    Article  CAS  PubMed  Google Scholar 

  10. Meldgaard Knudsen L, Jensen L, Gaarsdal E et al. A comparative study of sequential priming and mobilisation of progenitor cells with rhG-CSF alone and high-dose cyclophosphamide plus rhG-CSF Bone Marrow Transplant 2000 26: 717 722

    Article  CAS  PubMed  Google Scholar 

  11. Koc ON, Gerson SL, Cooper BW et al. Randomized cross-over trial of progenitor-cell mobilization: high-dose cyclophosphamide plus granulocyte colony-stimulating factor (G-CSF) versus granulocyte–macrophage colony-stimulating factor plus G-CSF Clin Oncol 2000 18: 1824 1830

    Article  CAS  Google Scholar 

  12. Kořístek Z, Mayer J . Bone marrow processing for transplantation using COBE Spectra cell separator J Hematother Stem Cell Res 1999 8: 443 448

    Article  PubMed  Google Scholar 

  13. Siena S, Bregni M, Brando B et al. Circulation of CD34+ hematopoietic stem cells in the peripheral blood of high-dose cyclophosphamide-treated patients: enhancement by intravenous recombinant human granulocyte–macrophage colony-stimulating factor Blood 1989 74: 1905 1914

    CAS  PubMed  Google Scholar 

  14. Mayer J, Kořístek Z, Vášová I et al. Ifosfamide and etoposide-based chemotherapy as salvage and mobilizing regimens for poor prognosis lymphoma Bone Marrow Transplant 1999 23: 413 419

    Article  CAS  PubMed  Google Scholar 

  15. Abrahamsen TG, Lange BJ, Packer RJ et al. A phase I and II trial of dose-intensified cyclophosphamide and GM-CSF in pediatric malignant brain tumors J Pediatr HematolOncol 1995 17: 134 139

    Article  CAS  Google Scholar 

  16. Pettengell R, Woll PJ, Thatcher R et al. Multicyclic, dose-intensive chemotherapy supported by sequential reinfusion of hematopoietic progenitors in whole blood J Clin Oncol 1995 13: 148 156

    Article  CAS  PubMed  Google Scholar 

  17. Díaz MA, Alegre A, Benito A et al. Peripheral blood progenitor cell collection by large-volume leukapheresis in low-weight children J Hematother 1998 7: 63 68

    Article  PubMed  Google Scholar 

  18. Alegre A, Díaz MA, Madero L et al. Large-volume leukapheresis for peripheral blood stem cell collection in children: a simplified single-apheresis approach Bone Marrow Transplant 1996 17: 923 927

    CAS  PubMed  Google Scholar 

  19. Madero L, Díaz MA, Benito A et al. Non-tunneled catheters for the collection and transplantation of peripheral blood stem cells in children Bone Marrow Transplant 1997 20: 53 56

    Article  CAS  PubMed  Google Scholar 

  20. Lasky LC, Bostrom B, Smith J . Clinical collection and use of peripheral blood stem cells in pediatric patients Transplantation 1989 47: 613 616

    Article  CAS  PubMed  Google Scholar 

  21. Takaue Y, Kawano Y, Abe T et al. Collection and transplantation of peripheral blood stem in very small children weighing 20 kg or less Blood 1995 86: 372 380

    CAS  PubMed  Google Scholar 

  22. Kajiume T, Yoshimi S, Kobayashi K, Kataoka N . Therelation between the number of PBSC and number ofleukaphereses in children J Hematother 1999 8: 185 188

    Article  CAS  PubMed  Google Scholar 

  23. Marson P, Petris MG, De Silvestro G . Collection of peripheral blood stem cells in pediatric patients: a concise review on technical aspects Bone Marrow Transplant 1998 22: (Suppl. 5) S7 11

    Article  PubMed  Google Scholar 

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Acknowledgements

This work was supported in part by the Research Grant of the Ministry of Health of the Czech Republic.

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Authors and Affiliations

  1. Department of Internal Medicine – Hematooncology, Masaryk University Hospital, Brno, Czech Republic

    Z Kořístek & J Mayer

  2. Department of Pediatric Oncology, Masaryk University Hospital, Brno, Czech Republic

    J Štěrba

  3. Tissue Bank, Masaryk University Hospital, Brno, Czech Republic

    D Havranová

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  1. Z Kořístek
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  2. J Štěrba
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  3. D Havranová
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  4. J Mayer
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Kořístek, Z., Štěrba, J., Havranová, D. et al. Technique for PBSC harvesting in children of weight under 10 kg. Bone Marrow Transplant 29, 57–61 (2002). https://doi.org/10.1038/sj.bmt.1703334

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