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CD34+ Cells

CD34+ cell collection efficiency does not correlate with the pre-leukapheresis hematocrit

Bone Marrow Transplantation volume 28, pages 597–601 (2001)Cite this article

Abstract

One hundred and seventy-seven large-volume leukapheresis procedures performed on 91 patients over a 15 month period were reviewed to see if the pre-apheresis hematocrit (Hct) affected the CD34+ cell collection efficiency (CE) of the Fenwal CS 3000 Plus cell separator. The Hct was 0.174–0.461 (median 0.317), and the peripheral blood CD34+ cell count 2–2487 per μl (median 21). The total CD34+ cell quantity collected was 3.0–2677.2 × 106 (median 113.0). Based on the number of CD34+cells contained in the blood volume processed (23.3–37303.2 × 106; median 318.0), the CE was 1.7–87.5% (median 30.3). No correlation was found between the Hct and CE (r2 = 0.0034; P = 0.44) or the total CD34+ cell quantity collected (r2 = 0.0040; P = 0.40). CEs for Hct <0.25 (median CE 36%), Hct 0.25–0.299 (median CE 30%) and Hct 0.30 (median CE 30%) were comparable. As expected, highly significant correlations were seen between the CD34+ cell quantities collected and quantities processed (r2 = 0.59; P < 10−6) as well as the peripheral blood CD34+ cell counts (r2 = 0.60; P < 10−6). We conclude that the minimum acceptable Hct or hemoglobin level for leukapheresis should be dictated by clinical circumstances because it does not affect stem cell collection. Bone Marrow Transplantation (2001) 28, 597–601.

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References

  1. Schmitz N, Linch DC, Dreger P et al. Randomised trial of filgrastim-mobilised peripheral blood progenitor cell transplantation versus autologous bone-marrow transplantation in lymphoma patients (published erratum appears in Lancet 1996; 347:914) Lancet 1996 347: 353–357

    Article  CAS  Google Scholar 

  2. Powles R, Mehta J, Kulkarni S et al. Allogeneic blood and bone-marrow stem-cell transplantation in haematological malignant diseases: a randomised trial Lancet 2000 355: 1231–1237

    Article  CAS  Google Scholar 

  3. Singhal S, Powles R, Treleaven J et al. A low CD34+ cell dose results in higher mortality and poorer survival after blood or marrow stem cell transplantation from HLA-identical siblings: should 2 × 106 CD34+ cells/kg be considered the minimum threshold? Bone Marrow Transplant 2000 26: 489–496

    Article  CAS  Google Scholar 

  4. Bensinger WI, Longin K, Appelbaum F et al. Peripheral blood stem cells (PBSCs) collected after recombinant granulocyte colony stimulating factor (rhG-CSF): an analysis of factors correlating with the tempo of engraftment after transplantation Br J Haematol 1994 87: 825–831

    Article  CAS  Google Scholar 

  5. Armitage S, Hargreaves R, Samson D, Brennan M, Kanfer E, Navarrete C . CD34 counts to predict the adequate collection of peripheral blood progenitor cells Bone Marrow Transplant 1997 20: 587–591

    Article  CAS  Google Scholar 

  6. Desikan KR, Barlogie B, Jagannath S et al. Comparable engraftment kinetics following peripheral-blood stem-cell infusion mobilized with granulocyte colony-stimulating factor with or without cyclophosphamide in multiple myeloma J Clin Oncol 1998 16: 1547–1553

    Article  CAS  Google Scholar 

  7. Singhal S, Mehta J, Desikan K et al. Collection of peripheral blood stem cells after a preceding autograft: unfavorable effect of prior interferon-α therapy Bone Marrow Transplant 1999 24: 13–17

    Article  CAS  Google Scholar 

  8. Shpall EJ, Champlin R, Glaspy JA . Effect of CD34+ peripheral blood progenitor cell dose on hematopoietic recovery Biol Blood Marrow Transplant 1998 4: 84–92

    Article  CAS  Google Scholar 

  9. Padley D, Strauss RG, Wieland M, Randels MJ . Concurrent comparison of the Cobe Spectra and Fenwal CS3000 for the collection of peripheral blood mononuclear cells for autologous peripheral stem cell transplantation J Clin Apheresis 1991 6: 77–80

    Article  CAS  Google Scholar 

  10. To LB, Stemmelin GR, Haylock DN et al. Collection efficiency on the Fenwal CS3000 when using filgrastim (recombinant methionyl human granulocyte colony-stimulating factor) as a peripheral blood stem cell mobilization agent J Clin Apheresis 1994 9: 17–20

    Article  CAS  Google Scholar 

  11. Mehta J, Powles R, Cabral S et al. Comparison of Cobe Spectra and Haemonetics MCS-3P cell separators for peripheral blood stem cell harvesting Bone Marrow Transplant 1995 16: 707–709

    CAS  PubMed  Google Scholar 

  12. Lin JS, Burgstaler EA, Pineda AA, Gertz MA . Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using Fenwal CS 3000 Plus. (Erratum in J Clin Apheresis 1995; 10:107) J Clin Apheresis 1995 10: 7–11

    Article  CAS  Google Scholar 

  13. Mehta J, Powles R, Treleaven J et al. Prospective, concurrent comparison of the Cobe Spectra and Haemonetics MCS-3P cell separators for leukapheresis after high-dose filgrastim in patients with hematologic malignancies J Clin Apheresis 1997 12: 63–67

    Article  CAS  Google Scholar 

  14. Stroncek DF, Clay ME, Smith J et al. Comparison of two blood cell separators in collecting peripheral blood stem cell components Transfus Med 1997 7: 95–99

    Article  CAS  Google Scholar 

  15. Menichella G, Lai M, Pierelli L et al. Evaluation of two different protocols for peripheral blood stem cell collection with the Fresenius AS 104 blood cell separator Vox Sang 1997 73: 230–236

    Article  CAS  Google Scholar 

  16. Mehta J, Powles R, Cabral S et al. A new automated apheresis system for the Cobe Spectra cell separator utilizing the platelet channel Blood 1997 90: (Suppl. 1) 331b

    Google Scholar 

  17. Schreiner T, Wiesneth M, Krug E et al. Collection of allogeneic peripheral blood progenitor cells by two protocols on an apheresis system Transfusion 1998 38: 1051–1055

    Article  CAS  Google Scholar 

  18. Ford CD, Pace N, Lehman C . Factors affecting the efficiency of collection of CD34-positive peripheral blood cells by a blood cell separator Transfusion 1998 38: 1046–1050

    Article  CAS  Google Scholar 

  19. Beretta F, van den Bosch S, Castelli D et al. Intrapatient comparison of an intermittent and a continuous flow cell separator for the collection of progenitor and stem cells from the blood Vox Sang 1998 75: 149–153

    Article  CAS  Google Scholar 

  20. Serafini R, Menichella G, Ciarli M et al. The application of two different blood cell separators to harvest CD34+ cells in patients suffering from non Hodgkin's lymphoma Int J Artif Organs 1999 22: 583–588

    Article  CAS  Google Scholar 

  21. Wilke R, Brettell M, Prince HM et al. Comparison of COBE Spectra software version 4.7 PBSC and version 6.0 auto PBSC program J Clin Apheresis 1999 14: 26–30

    Article  CAS  Google Scholar 

  22. Kulkarni S, Powles R, Mehta J et al. Feasibility of donating both marrow and blood cells within a week in normal donors Blood 1997 90: (Suppl. 1) 328b

    Google Scholar 

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Authors and Affiliations

  1. Division of Hematology/Oncology, Department of Internal Medicine, The Hematopoietic Stem Cell Transplant Program, Northwestern University Medical School and The Robert H Lurie Comprehensive Cancer Center of Northwestern University, Chicago, IL, USA

    J Mehta, Y Oyama, J Winter, S Williams, M Tallman, S Singhal, M Villa, T Shook, G Soff, S Masarik, G Ramsey & L Gordon

  2. Division of Immunotherapy and Autoimmune Diseases, Department of Internal Medicine, Northwestern University Medical School, Chicago, IL, USA

    R Burt & A Traynor

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  1. J Mehta
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Mehta, J., Oyama, Y., Winter, J. et al. CD34+ cell collection efficiency does not correlate with the pre-leukapheresis hematocrit. Bone Marrow Transplant 28, 597–601 (2001). https://doi.org/10.1038/sj.bmt.1703197

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