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Post-Transplant Complications

Altered expression of receptors for thyroid hormone and insulin-like growth factor-1 during reconstitution after allogeneic hematopoietic stem cell transplantation

Bone Marrow Transplantation volume 27, pages 1163–1171 (2001)Cite this article

Abstract

Treatment with neuroendocrine hormones has been suggested to promote reconstitution of the immune system after hematopoietic stem cell transplantation (HSCT). We investigated the expression of genes encoding receptors for growth hormone (GH), insulin-like growth factor-1 (IGF-1) and triiodothyronine (T3), at various time points after HSCT in 16 patients and 15 healthy controls. Peripheral blood mononuclear cells were isolated and RNA for GH receptor (GHR), IGF-I receptor (IGF-IR) and thyroid hormone receptor (TRα1) was amplified by RT-PCR. The expression of the genes was compared with the expression of β-actin. We demonstrate increased expression of TRα1 RNA in patients at 1.5 months post HSCT, compared to a group of healthy controls, and decreased expression of IGF-IR RNA at 2 and 3 months post HSCT, compared to the controls. Serum from three of the patients was also analyzed for levels of T3, T4, TSH and IGF-1 at several time points after HSCT. Serum levels for T3, thyroxine (T4), thyroid stimulating hormone (TSH) and IGF-1 were within the normal range in all samples. Our results on the molecular level indicate a role for thyroid hormones and IGF-I in immune reconstitution after HSCT, even though the serum levels of T3, T4, TSH and IGF-I are normal. Bone Marrow Transplantation (2001) 27, 1163–1171.

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Acknowledgements

We would like to thank the staff at the Centre for Allogeneic Stem cell Transplantation for excellent patient care. We are grateful to S Öman and C Tammik for providing us with the sample material and to Dr O Ringdén for patient care, clinical data and critical reading of the manuscript. This work was supported by grants from the Swedish Medical Research Council (K99–06X-11599), the Ake Wiberg Foundation and the Karolinska Institute.

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Authors and Affiliations

  1. Department of Surgery, Arvid Wretlind Laboratory for Metabolic Research, Huddinge University Hospital, Stockholm, Sweden

    B Omazic, J Permert & I Lundkvist

  2. Division Biovitrum, Pharmacia AB, Stockholm, Sweden

    I Näsman-Björk

  3. Division of Clinical Immunology, Department of Immunology, Microbiology, Pathology and Infectious Disease, Huddinge University Hospital, Stockholm, Sweden

    J Johansson, P Hentschke & J Mattsson

  4. Center for Allogeneic Stem cell Transplantation, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden

    P Hentschke & J Mattsson

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Omazic, B., Näsman-Björk, I., Johansson, J. et al. Altered expression of receptors for thyroid hormone and insulin-like growth factor-1 during reconstitution after allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant 27, 1163–1171 (2001). https://doi.org/10.1038/sj.bmt.1703062

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