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Cord Blood Stem Cells

Collection, processing and cryopreservation of umbilical cord blood for unrelated transplantation

Bone Marrow Transplantation volume 26, pages 1263–1270 (2000)Cite this article

Abstract

Umbilical cord blood (UCB) transplantation is being used as an alternative source of hematopoietic stem cells for bone marrow reconstitution. Separation and processing of UCB samples in large numbers for storage in cord blood banks ideally needs to be partially automated. The aim of this study was to establish and standardize a method for unrelated cord blood banking as well as the biological characterization of the samples. Up to October 1999, a total of 938 UCB units (mean volume 84.6 ± 23.6 ml, nucleated cell (NC) count 0.90 ± 0.37 × 109, total CFU-GM 79 ± 72 × 104, CD34+ cell count 2.46 ± 2.72 × 106) had been collected. Twenty-three per cent of all UCB samples had a NC count below 0.4 × 109 and were discarded. The initial bacterial contamination rate was reduced to less than 5% as a result of extensive training in collection procedures. Using a modification of a triple bag system and adding a solution of 6% hydroxyethyl starch, the UCB was separated by two centrifugation steps into three components: buffy coat, red cell and plasma fractions. The overall recoveries for NC, CFU-GM and CD34+ cells were 87.4 ± 8.5%, 88.8 ± 6.6% and 90.3 ± 12.4%, respectively, in a mean final volume of 27 ± 4.2 ml. Bone Marrow Transplantation (2000) 26, 1263–1270.

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Acknowledgements

We would like to acknowledge the staff of the Hospitals Clínico Universitario, Xeral Cíes and Arquitecto Marcide for their collaboration in the collection of the umbilical cord blood.

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  1. N M-Reboredo and A Díaz: Both authors contributed equally to the work

Authors and Affiliations

  1. Department of Cryobiology, Galician Blood Transfusion Center, Santiago de Compostela, Spain

    A Castro & RG Villaescusa

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  1. N M-Reboredo
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  2. A Díaz
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  3. A Castro
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  4. RG Villaescusa
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M-Reboredo, N., Díaz, A., Castro, A. et al. Collection, processing and cryopreservation of umbilical cord blood for unrelated transplantation. Bone Marrow Transplant 26, 1263–1270 (2000). https://doi.org/10.1038/sj.bmt.1702728

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