Abstract
Lethally irradiated mice were grafted with syngeneic bone marrow cells or left ungrafted. Mice of each group were injected with different hematopoietic cytokines for 5 consecutive days starting immediately after irradiation or left uninjected. The recovery of lymphoid tissues induced by hematopoietic cytokines 7 days after irradiation and bone marrow cell transplantation was comparable to that observed at days 21–28 in irradiated, bone marrow-grafted, but cytokine-uninjected mice. IL-11 or IL-6, in combination with IL-3, was able to hasten thymus, spleen and blood cell numbers and functions. SCF also displayed a detectable effect when used with IL-3. Conversely, the IL-6 super-agonist K-7/D-6 was able, when injected alone, to induce significant recovery of thymus, spleen and blood cells. Thus, K-7/D-6 appears to be a most efficient cytokine for fast reconstitution of lymphoid tissues after irradiation and bone marrow transplantation. Bone Marrow Transplantation (2000) 25, 427–433.
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Acknowledgements
We thank Dr S Neben (Genetics Institute) for kindly providing IL-11, and Dr G Ciliberto (IRBMP Angeletti) for the gifts of hu r IL-6, K-7/D-6 and human soluble IL-6R. This work was supported in part by CNR Progetto Finalizzato Biotecnologie.
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Frasca, D., Guidi, F., Arbitrio, M. et al. Hematopoietic reconstitution after lethal irradiation and bone marrow transplantation: effects of different hematopoietic cytokines on the recovery of thymus, spleen and blood cells. Bone Marrow Transplant 25, 427–433 (2000). https://doi.org/10.1038/sj.bmt.1702169
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DOI: https://doi.org/10.1038/sj.bmt.1702169
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