Administration of G-CSF to normal individuals diminishes L-selectin⁺ T cells in the peripheral blood that respond better to alloantigen stimulation than L-selectin⁻ T cells

Abstract

To determine whether administration of G-CSF induces phenotypic or functional changes in T cells, we examined peripheral blood T cells from normal individuals receiving G-CSF for activation antigen and adhesion molecule expression before and after G-CSF administration. G-CSF (10 μg/kg/day) was administered subcutaneously to 14 normal individuals for 3–5 days and their PBMC were serially analyzed with monoclonal Ab (mAb) directed to HLA-DR, CD45RO, CD45RA, CD25, CD122, CD95, CD11a, CD49d, CD44 and CD62L (L-selectin) coupled with anti-CD3 mAb. Among T cells positive for these antigens, only the proportion of T cells expressing L-selectin significantly decreased from 68% to 37% after 3-day G-CSF administration. When peripheral blood CD3⁺ T cells obtained before and after G-CSF administration were sorted into two populations depending on the expression of L-selectin and tested for their proliferative response to allogeneic B cells, the reactivity of L-selectin⁻ cells to alloantigen stimulation was consistently lower than that of L-selectin⁺ cells regardless of the exposure to G-CSF. The decrease in the relative number of L-selectin⁺ cells induced by G-CSF administration may contribute to the unexpectedly low incidence of severe acute GVHD after allogeneic PBSC transplantation.