Abstract
A MOSAIC virus of sannhemp (Crotalaria juncea Linn.) was crystallized1 at the Indian Agricultural Research Institute following the method suggested by Bawden2. Later, the virus was crystallized, employing the method of Markham and Smith3 used for isolation and crystallization of turnip yellow mosaic virus. Control experiments conducted with the expressed sap of the leaves of healthy sannhemp plants and subjected to treatment similar to that given to the infective sap showed that no such crystalline substance could be obtained from healthy material. Solutions of the crystalline preparation indicated positive results in every inoculation test, and typical symptoms were produced on sannhemp plants. Raychaudhuri and Ramamoorty4 studied the light-absorption curve of 0.11 per cent crystalline solution of sannhemp mosaic virus in a centimetre layer of the solution on a Hilger Spekker spectro-photometer E2, and observed two maxima, at 2770 A. and 2632 A., and a third one at 2958 A., with an inflexion point at about 2146 A. A preliminary study has now been made of the size and shape of the structures considered to be virus particles, as observed in the electron-micrographs of the crystalline preparation. The micrographs have been made with the electron microscope installed in the Institute of Nuclear Physics at Calcutta5.
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DAS GUPTA, N., DE, M. & RAYCHAUDHURI, S. Structure of Sannhemp (Crotalaria juncea Linn.) Mosaic Virus with the Electron Microscope. Nature 168, 114 (1951). https://doi.org/10.1038/168114a0
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DOI: https://doi.org/10.1038/168114a0
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