Abstract
THE ammoniacal silver nitrate spray1 used for the detection of sugars has several disadvantages; to those mentioned by Partridge2 should be added the necessity for very careful control of the heating step, particularly important in laboratories lacking special apparatus. We have introduced modifications, based on a test given by Feigl3 for reducing sugars, which eliminate the heating step, and in which the reagents are applied in organic solvents, thus removing the danger of migration of the sugar spots. The method has been in use for more than a year, and has proved easy to handle and extremely reliable.
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References
Partridge, S. M., Biochem. J., 42, 238 (1948).
Partridge, S. M., Nature, 164, 443 (1949).
Feigl, F., “Quantitative Analysis by Spot Tests”, 3rd English edit. (New York–Amsterdam: Elsevier, 1947.)
Seidell, A., “Solubilities of Organic Compounds”, 3rd edit. (New York: Van Nostrand co., 1941.)
Hough, L., Nature, 165, 400 (1950).
Mattson, A. M., and Jensen, C. O., Anal. Chem., 22, 182 (1950).
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TREVELYAN, W., PROCTER, D. & HARRISON, J. Detection of Sugars on Paper Chromatograms. Nature 166, 444–445 (1950). https://doi.org/10.1038/166444b0
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DOI: https://doi.org/10.1038/166444b0
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