Purification of Anti-pernicious Anæmia Factors from Liver

Abstract

WE have now prepared from ox liver two red pigments both highly active in pernicious anæmia. The crude extract was purified by the methods employed by Emery, and Parker¹ and then by repeated chromatography. Full details of these techniques will be offered for publication later. Recently we have used as starting material a proteolysed liver extract made on a manufacturing scale ; this has given better yields of a product differing slightly in chromatographic behaviour, and of higher activity. Some preparations of both types have been separated by partition chromatography into two red fractions ; the proportions suggest that one arises from the other by proteolysis.