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  • Original Article
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Kinetics of plasma apolipoprotein C-III as a determinant of diet-induced changes in plasma triglyceride levels

European Journal of Clinical Nutrition volume 62, pages 10–17 (2008)Cite this article

Abstract

Objective:

To compare the effect of a high monounsaturated fatty acid (MUFA) diet and of a control low-fat diet consumed under ad libitum conditions on plasma apolipoprotein (apo) C-III metabolism.

Design:

Randomized, two-arm parallel dietary trial.

Setting:

Diets were prepared and consumed at the metabolic kitchen of the Department of Food Sciences and Nutrition, and laboratory analyses were performed at the Institute of Nutraceuticals and Functional Foods at Laval University.

Subjects and interventions:

Eighteen men were randomly assigned to either the high MUFA diet or the low-fat control diet, which they consumed for 6–7 weeks. Before and after the dietary intervention, subjects received a primed-constant infusion of [5,5,5-D3]-L-leucine for 12 h under constant feeding conditions for the determination of plasma apoC-III kinetics.

Results:

The high-MUFA diet and the low-fat control diet had no significant impact on plasma apoC-III production rate (PR) or fractional catabolic rate. However, diet-induced variations in plasma apoCIII PR predicted the reduction in plasma triglycerides and apoC-III levels (r=0.85, P<0.01 and r=0.73, P<0.05, respectively) in the high MUFA group only.

Conclusions:

These results suggest that the hypotriglyceridemic effect of a high-MUFA diet may be attributable in part to a reduced hepatic production of apoC-III.

Sponsorship:

This study was supported in part by an operating grant from the Canadian Institutes of Health Research (CIHR), and the Canada Research Chair in Nutrition and Cardiovascular Health (B Lamarche).

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Acknowledgements

We express our gratitude to the study participants for their invaluable contribution, to Louise Corneau for her dedicated work and to Benjamin Ware, Josée Lévesque and Raphaëlle Verge for their contribution to laboratory work. SD is the recipient of a Canada Graduate Scholarship Doctoral Award from the Canadian Institutes of Health Research (CIHR) and of a Studentship from the Fonds de la Recherche en Santé du Québec (FRSQ). M-EP received a doctoral Studentship from the FRSQ. PC is a Clinical Scholar from the FRSQ. BL is Chair Professor in Nutrition and Cardiovascular Health from the Canada Research Chair Program.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Institute of Nutraceuticals and Functional Foods, Laval University, Québec, Canada

    S Desroches, I L Ruel, M-E Paradis, W R Archer & B Lamarche

  2. Department of Anatomy and Physiology, Faculty of Medicine, Laval Hospital Research Center, Laval University, Québec, Canada

    Y Deshaies

  3. Department of Medicine, Faculty of Medicine, Lipid Research Center, CHUL Research Center, Québec, Canada

    P Couture

  4. College of Pharmacy, Touro University-California, Vallejo, CA, USA

    N Bergeron

Authors
  1. S Desroches
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  2. I L Ruel
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  3. Y Deshaies
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  4. M-E Paradis
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  5. W R Archer
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  6. P Couture
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  7. N Bergeron
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  8. B Lamarche
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Corresponding author

Correspondence to B Lamarche.

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Guarantor: B Lamarche.

Contributors: SD was responsible for the kinetic and statistical analyses and wrote the manuscript under the supervision of BL. ILR was responsible for the apolipoprotein isotopic enrichment determinations. M-EP performed the analyses pertaining to lipoprotein and hepatic lipase activities in the laboratory of YD. WRA managed the dietary intervention and monitored the participants during the study. PC was responsible for the screening and medical supervision of the study participants. NB designed the intervention. All authors provided a critical review of the manuscript.

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Desroches, S., Ruel, I., Deshaies, Y. et al. Kinetics of plasma apolipoprotein C-III as a determinant of diet-induced changes in plasma triglyceride levels. Eur J Clin Nutr 62, 10–17 (2008). https://doi.org/10.1038/sj.ejcn.1602673

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