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Two variants in the resistin gene and the response to long-term overfeeding

European Journal of Clinical Nutrition volume 58, pages 654–659 (2004)Cite this article

Abstract

Objective and subjects: To investigate the role of resistin gene variants on the adiposity and metabolic changes observed in response to a 100-day overfeeding protocol conducted with 12 pairs of monozygotic twins.

Measurements: Body-fat measurements included hydrodensitometry and abdominal fat from computed tomography. Plasma glucose and insulin during fasting and in response to an oral glucose tolerance test (OGTT) were assayed. A 4.2 MJ test meal was consumed, after which calorimetric measurements were performed for 240 min.

Results: Respiratory quotient (RQ) decreased (P=0.001) more in AA/AG than in GG subjects of the IVS2+181G>A polymorphism after the caloric surplus and the significance persisted when correction for multiple testing was performed. Total abdominal (P=0.027) and visceral (P=0.004) fat increased more in TC than in TT subjects of the IVS2+39C>T polymorphism. In response to overfeeding, glucose area under the curve during the OGTT showed a slight decrease (P=0.031) in the TC while it increased in TT subjects. OGTT insulin area tended to increase less (P=0.055) in TC than in TT subjects. After overfeeding, fasting insulin was lower in TC than in TT subjects (P=0.010). In addition, TC subjects experienced more decrease in RQ than TT subjects (P=0.034).

Conclusion: The IVS2+181G>A variant was associated with the changes in RQ in response to overfeeding. The IVS2+39C>T polymorphism was associated with overfeeding-induced changes in abdominal visceral fat, OGTT glucose area and RQ. The results suggest that sequence variation in the resistin gene is involved in the adaptation to chronic positive energy balance.

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Acknowledgements

We are indebted to Jacques Bouillon, Suzie Hamel, Brigitte Zément, Maryse Lebrun, Martine Marcotte, Monique Chagnon, Josée Lapointe, Henri Bessette, Gilles Bouchard, Heidi Häikiö and Serge Carbonneau for their contributions to this study. Special thanks to Guy Fournier and Dr Germain Thériault for their role in the management of the study. Gratitude is expressed to Dr Andre Nadeau and the staff of the Diabetes Research Unit for the glucose and insulin assays. Supported in part by a grant (DK 34624) from the National Institutes of Health, by the Medical Council of the Academy of Finland and the Finnish Foundation for Cardiovascular Research. C Bouchard is supported in part by the George A Bray Chair in Nutrition.

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Authors and Affiliations

  1. Department of Internal Medicine and Biocenter Oulu, University of Oulu, Oulu, Finland

    O Ukkola & Y Antero Kesäniemi

  2. Physical Activity Sciences Laboratory, Laval University, Québec, QC, Canada

    A Tremblay

  3. Pennington Biomedical Research Center, Baton Rouge, LA, USA

    C Bouchard

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  1. O Ukkola
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Ukkola, O., Antero Kesäniemi, Y., Tremblay, A. et al. Two variants in the resistin gene and the response to long-term overfeeding. Eur J Clin Nutr 58, 654–659 (2004). https://doi.org/10.1038/sj.ejcn.1601861

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