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The p53-inducible apoptotic protein AMID is not required for normal development and tumor suppression

Oncogene volume 25, pages 849–856 (2006)Cite this article

Abstract

AMID is an apoptosis-inducing factor (AIF)-homologous and mitochondria-associated protein that has been implicated in caspase-independent apoptosis. Transcription of human AMID gene is upregulated by p53 and downregulated in tumors in comparison to their matched normal tissues, suggesting the possibility that AMID is involved in the downstream effects of p53. To investigate the physiological functions of AMID, we generated AMID-deficient mice by gene targeting. AMID-deficient mice are viable and fertile, develop normally and lack obvious phenotypic changes compared to wild-type mice up to 1 year old. AMID−/− mice up to 1 year old have no spontaneous tumors and show similar fibrosarcoma incidence after MCA inoculation compared to wild-type mice. AMID−/− embryonic fibroblasts exhibit normal proliferation but slightly increased resistance to genotoxin-induced growth arrest. These findings suggest that AMID is not required for normal development and p53-mediated tumor suppression.

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Acknowledgements

We thank Dr Raul Torres for help with gene knockout studies, Dr Gwinevere Murphy for providing us anti-p53 antibody and Dr Stephen J Elledge for providing the Recombination Cloning System.This work was supported by a grant from the NIH (CA108771).

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Authors and Affiliations

  1. National Jewish Medical and Research Center, Denver, CO, USA

    J Mei, S Webb, B Zhang & H-B Shu

  2. College of Life Sciences, Wuhan University, Wuhan, China

    H-B Shu

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  1. J Mei
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  2. S Webb
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Correspondence to H-B Shu.

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Mei, J., Webb, S., Zhang, B. et al. The p53-inducible apoptotic protein AMID is not required for normal development and tumor suppression. Oncogene 25, 849–856 (2006). https://doi.org/10.1038/sj.onc.1209121

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