Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Short Communication
  • Published:

Overexpression of CEBPβ correlates with decreased TFF1 in gastric cancer

Oncogene volume 25, pages 643–649 (2006)Cite this article

Abstract

CCAAT element binding protein beta (C/EBPβ) is an important regulator of cell growth, differentiation and in promoting tumor invasiveness. C/EBPβ is located on chromosome 20q, which is amplified in many solid tumors including gastric cancers (GC). We sought to characterize the status of C/EBPβ expression in GCs, which was recently found to repres TFF1 gene. Microarray analysis revealed overexpression of C/EBPβ in 25 of 27 (93%) GC when compared to 12 normal gastric tissue samples. RT–PCR analysis confirmed the overexpression of C/EBPβ transcripts in 54 of 59 (91%) GC. In total, 15 of 18 gastric tumors exhibited at least fivefold higher C/EBPβ transcript levels compared to their corresponding adjacent normal gastric tissue samples. Moreover, immunohistochemistry analysis demonstrated increased nuclear staining of C/EBPβ in 10 of 13 GC and at least fourfold overexpression of C/EBPβ in three primary GC compared to adjacent normal gastric tissue. Furthermore, a striking correlation of decreased TFF1 expression with increased C/EBPβ was observed in the gastric tumors studied. Microarray analysis demonstrated a loss of TFF1 expression in all 27 GC cases examined, of which 25 exhibited high C/EBPβ expression compared to normal gastric tissue. RT–PCR analysis revealed loss of TFF1 expression in 56 of 59 gastric tumors in which 54 of these tumors exhibited overexpression of C/EBPβ. Immunohistochemical analysis revealed overexpression of C/EBPβ in 10 of 13 gastric tumors that exhibited low expression of TFF1 at the protein level. Thus, overexpression of the transcription factor C/EBPβ in the majority of GCs is a novel finding.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

References

  • Beckler AD, Roche JK, Harper JC, Petroni G, Frierson HF, Moskaluk CA et al. (2003). Cancer 98: 2184–2191.

  • Bundy LM, Sealy L . (2003). Oncogene 22: 869–883.

  • Dossinger V, Kayademir T, Blin N, Gott P . (2002). Cell Physiol Biochem 12: 197–206.

  • El-Rifai W, Frierson HF, Moskaluk CA, Harper JC, Petroni GR, Bissonette EA et al. (2001). Gastroenterology 121: 592–598.

  • Gorgoni B, Caivano M, Arizmendi C, Poli V . (2001). J Biol Chem 276: 40769–40777.

  • Gutierrez S, Javed A, Tennant DK, van Rees M, Montecino M, Stein GS et al. (2002). J Biol Chem 277: 1316–1323.

  • Hasegawa S, Furukawa Y, Li M, Satoh S, Kato T, Watanabe T et al. (2002). Cancer Res 62: 7012–7017.

  • Judd LM, Alderman BM, Howlett M, Shulkes A, Dow C, Moverley J et al. (2004). Gastroenterology 126: 196–207.

  • Kagan BL, Henke RT, Cabal-Manzano R, Stoica GE, Nguyen Q, Wellstein A et al. (2003). Cancer Res 63: 1696–1705.

  • Kang KW, Cho IJ, Lee CH, Kim SG . (2003). J Natl Cancer Inst 95: 53–66.

  • Kintscher J, Yamkamon V, Braas D, Klempnauer KH . (2004). Oncogene 23: 5807–5814.

  • Kowenz-Leutz E, Leutz A . (1999). Mol Cell 4: 735–743.

  • Lamb J, Ramaswamy S, Ford HL, Contreras B, Martinez RV, Kittrell FS et al. (2003). Cell 114: 323–334.

  • Lefebvre O, Chenard MP, Masson R, Linares J, Dierich A, LeMeur M et al. (1996). Science 274: 259–262.

  • Li W, Kessler P, Yeger H, Alami J, Reeve AE, Heathcott R et al. (2005). Cancer Res 65: 2592–2601.

  • Oya M, Horiguchi A, Mizuno R, Marumo K, Murai M . (2003). Clin Cancer Res 9: 1021–1027.

  • Rask K, Thorn M, Ponten F, Kraaz W, Sundfeldt K, Hedin L et al. (2000). Int J Cancer 86: 337–343.

  • Sankpal NV, Mayo MW, Powell SM . (2005). Biochim Biophys Acta 1728: 1–10.

  • Sundfeldt K, Ivarsson K, Carlsson M, Enerback S, Janson PO, Brannstrom M et al. (1999). Br J Cancer 79: 1240–1248.

  • Tebbutt NC, Giraud AS, Inglese M, Jenkins B, Waring P, Clay FJ et al. (2002). Nat Med 8: 1089–1097.

  • Vinson CR, Sigler PB, McKnight SL . (1989). Science 246: 911–916.

  • Welsh JB, Zarrinkar PP, Sapinoso LM, Kern SG, Behling CA, Monk BJ et al. (2001). Proc Natl Acad Sci USA 98: 1176–1181.

  • Zahnow CA, Younes P, Laucirica R, Rosen JM . (1997). J Natl Cancer Inst 89: 1887–1891.

Download references

Acknowledgements

This work was supported by grant awards from the National Cancer Institute: CA67900 (SM Powell). The contents of this work are solely our responsibility, and do not necessarily represent the official views of the NCI or the University of Virginia. We also thank Jeffrey Harper for technical help.

Author information

Authors and Affiliations

  1. Department of Internal Medicine, University of Virginia Health System, Charlottesville, VA, USA

    N V Sankpal & S M Powell

  2. Department of Pathology, University of Virginia Health System, Charlottesville, VA, USA

    C A Moskaluk

  3. Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA

    G M Hampton

Authors
  1. N V Sankpal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C A Moskaluk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G M Hampton
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S M Powell
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S M Powell.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sankpal, N., Moskaluk, C., Hampton, G. et al. Overexpression of CEBPβ correlates with decreased TFF1 in gastric cancer. Oncogene 25, 643–649 (2006). https://doi.org/10.1038/sj.onc.1209081

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1209081

Keywords

This article is cited by

Search

Advanced search

Quick links