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THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma

Oncogene volume 24, pages 6525–6532 (2005)Cite this article

Abstract

Using oligonucleotide microarray analysis, THY1, mapping close to a previously defined 11q22–23 nasopharyngeal carcinoma (NPC) critical region was identified as showing consistent downregulated expression in the tumour segregants, as compared to their parental tumour-suppressing microcell hybrids (MCHs). Gene expression and protein analyses show that THY1 was not expressed in the NPC HONE1 recipient cells, tumour segregants, and other NPC cell lines; THY1 was exclusively expressed in the non-tumourigenic MCHs. The mechanism of THY1 gene inactivation in these cell lines was attributed to hypermethylation. Clinical study showed that in 65% of NPC specimens there was either downregulation or loss of THY1 gene expression. Using a tissue microarray and immunohistochemical staining, 44% of the NPC cases showed downregulated expression of THY1 and 9% lost THY1 expression. The frequency of THY1 downregulated expression in lymph node metastatic NPC was 63%, which was significantly higher than in the primary tumour (33%). After transfection of THY1 gene into HONE1 cells, a dramatic reduction of colony formation ability was observed. These findings suggest that THY1 is a good candidate tumour suppressor gene in NPC, which is significantly associated with lymph node metastases.

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Acknowledgements

We acknowledge the financial support from the Research Grants Council of the Hong Kong Special Administrative Region, People's Republic of China: Grant numbers HKUST 6113/01M, CA99/00.SC02, and CA03/04.SC01 to MLL, the Agency for Science Technology and Research, Singapore to EL, and the Swedish Cancer Society, the Swedish Research Council, STINT, the Swedish Institute, the Royal Swedish Academy of Sciences and INTAS to ERZ.

Author information

Author notes

  1. Dan Xie

    Present address: Cancer Institute, Sun Yat-Sen University, Guangzhou, People's Republic of China

  2. Yue Cheng

    Present address: Genetics Branch, National Cancer Institute, National Naval Medical Center, Bethesda, MD, 20889-5105, USA

  3. Mande Kuppusamy Kumaran

    Present address: Physiological and Genomics Medicine Group, Imperial College, London, W12 ONN, UK

Authors and Affiliations

  1. Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong (SAR), People's Republic of China

    Hong Lok Lung, Arthur Kwok Leung Cheung, Yue Cheng & Maria Li Lung

  2. Genome Institute of Singapore, Biomedical Sciences Institutes, Singapore

    Dhinoth Kumar Bangarusamy, Mande Kuppusamy Kumaran, Lance Miller & Edison Tak-Bun Liu

  3. The Department of Clinical Oncology, University of Hong Kong, Pokfulam, Hong Kong (SAR), People's Republic of China

    Dan Xie, Xin-Yuan Guan & Jonathan Shuntong Sham

  4. Cancer Institute, Sun Yat-Sen University, Guangzhou, People's Republic of China

    Yan Fang

  5. Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, 14642, NY, USA

    Liqiong Li & Nancy Wang

  6. Microbiology and Tumor Biology Center, Center for Genomics and Bioinformatics, Karolinska Institute, Stockholm, Sweden

    Alexey I Protopopov & Eugene R Zabarovsky

  7. Department of Anatomy, University of Hong Kong, Pokfulam, Hong Kong (SAR), People's Republic of China

    Sai Wah Tsao

  8. Department of Microbiology and Molecular Genetics, University of California, Irvine, 92697, CA, USA

    Eric J Stanbridge

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  1. Hong Lok Lung
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  18. Maria Li Lung
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Correspondence to Maria Li Lung.

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Lung, H., Bangarusamy, D., Xie, D. et al. THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma. Oncogene 24, 6525–6532 (2005). https://doi.org/10.1038/sj.onc.1208812

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  • DOI: https://doi.org/10.1038/sj.onc.1208812

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