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Mutation frequencies and AID activation state in B-cell lymphomas from Ung-deficient mice

Oncogene volume 24, pages 3063–3066 (2005)Cite this article

Abstract

B-cell lymphomas arising in lymph nodes and spleen of aging mice deficient in the Ung DNA glycosylase were recovered, dispersed, grown in short-term culture, and CD19-positive B-cells retrieved and analysed. Several tumors as well as controls only expressed detectable amounts of the Aid deaminase after mitogenic stimulation, as estimated by real-time PCR of transcripts. However, one unusually large lymph node tumor expressed a high level of Aid constitutively. This particular tumor also showed a substantially increased mutation frequency in the Aid gene itself as well as in the bcl-6 and c-myc genes, but not in the p53 gene, consistent with aberrant somatic hypermutation. Other B-cell lymphomas from Ung−/− mice exhibited a modest increase in mutation frequency.

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Acknowledgements

We thank Ashfaq Gilkar and Gordon Stamp for histopathology, Paul AW Edwards and Mira Grigorova for help with spectral karyotyping, and Geza Hrivnak and Del Watling for tumor collection. This work was supported by Cancer Research UK.

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  1. Hilde Nilsen

    Present address: University of Oslo, Biotechnology Centre, 0317, Oslo, Norway

  2. Hilde Nilsen and Qian An: These authors contributed equally to this work

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  1. Clare Hall Laboratories, CR-UK London Research Institute, South Mimms, Hertfordshire, EN6 3LD, UK

    Hilde Nilsen, Qian An & Tomas Lindahl

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  1. Hilde Nilsen
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Correspondence to Tomas Lindahl.

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Nilsen, H., An, Q. & Lindahl, T. Mutation frequencies and AID activation state in B-cell lymphomas from Ung-deficient mice. Oncogene 24, 3063–3066 (2005). https://doi.org/10.1038/sj.onc.1208480

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