Abstract
AMID, also called PRG3, is an AIF-homologous and mitochondria-associated protein that has been implicated in caspase-independent apoptosis. In this report, we demonstrated that human AMID gene promoter was activated by p53 in reporter gene assays. Chromatin immunoprecipitation experiments indicated that p53 could bind to human AMID promoter. Deletion mutagenesis indicated that human AMID promoter contains two p53-responsive elements. Furthermore, expression array analysis indicated that human AMID mRNA expression was downregulated in a majority of human tumors. Our findings point to the possibility that AMID is a p53-downstream gene involved in tumorigenesis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Amarante-Mendes GP, Finucane DM, Martin SJ, Cotter TG, Salvesen GS and Green DR . (1998). Cell Death Differ., 5, 298–306.
Bidere N and Senik A . (2001). Apoptosis, 6, 371–375.
Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA and Gruss P . (1998). Cell, 94, 727–737.
Daugas E, Susin SA, Zamzami N, Ferri KF, Irinopoulou T, Larochette N, Prevost MC, Leber B, Andrews D, Penninger J and Kroemer G . (2000). FASEB J., 14, 729–739.
el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW and Vogelstein B . (1993). Cell, 75, 817–825.
Green DR and Reed JC . (1999). Science, 281, 1309–1312.
Hakem R, Hakem A, Duncan GS, Henderson JT, Woo M, Soengas MS, Elia A, de la Pompa JL, Kagi D, Khoo W, Potter J, Yoshida R, Kaufman SA, Lowe SW, Penninger JM and Mak TW . (1998). Cell, 94, 339–352.
Haraguchi M, Torii S, Matsuzawa S, Xie Z, Kitada S, Krajewski S, Yoshida H, Mak TW and Reed JC . (2000). J. Exp. Med., 191, 1709–1720.
Hengartner MO . (2000). Nature, 407, 770–776.
Hunot S and Flavell RA . (2001). Science, 292, 865–866.
Joza N, Susin SA, Daugas E, Stanford WL, Cho SK, Li CY, Sasaki T, Elia AJ, Cheng HY, Ravagnan L, Ferri KF, Zamzami N, Wakeham A, Hakem R, Yoshida H, Kong YY, Mak TW, Zuniga-Pflucker JC, Kroemer G and Penninger JM . (2001). Nature, 410, 549–554.
Krause K, Haugwitz U, Wasner M, Wiedmann M, Mossner J and Engeland K . (2001). Biochem. Biophys. Res. Commun., 284, 743–750.
Kuida K, Haydar TF, Kuan CY, Gu Y, Taya C, Karasuyama H, Su MS, Rakic P and Flavell RA . (1998). Cell, 94, 325–337.
Kuida K, Zheng TS, Na S, Kuan C, Yang D, Karasuyama H, Rakic P and Flavell RA . (1996). Nature, 384, 368–372.
Levine AJ . (1997). Cell, 88, 323–331.
Levine AJ, Momand J and Finlay CA . (1991). Nature, 351, 453–456.
Li K, Li Y, Shelton JM, Richardson JA, Spencer E, Chen ZJ, Wang X and Williams RS . (2000). Cell, 101, 389–399.
Loeffler M, Daugas E, Susin SA, Zamzami N, Metivier D, Nieminen AL, Brothers G, Penninger JM and Kroemer G . (2001). FASEB J., 15, 758–767.
Lutzker SG and Levine AJ . (1996). Nat. Med., 2, 804–810.
Miramar MD, Costantini P, Ravagnan L, Saraiva LM, Haouzi D, Brothers G, Penninger JM, Peleato ML, Kroemer G and Susin SA . (2001). J. Biol. Chem., 276, 16391–16398.
Ohiroa Y, Garkavtsevb I, Kobayashif S, Sreekumarc KR, Nantzf R, Higashikubof BT, Duya SL, Higashikubof R, Ushevaa A, Giusd D, Kleye N and Horikoshif N . (2002). FEBS Lett., 524, 163–171.
Oren M . (2003). Cell Death Diffier., 10, 431–442.
Resnick-Silverman L, St Clair S, Maurer M, Zhao K and Manfredi JJ . (1998). Genes Dev., 12, 2102–2107.
Shang Y, Hu X, DiRenzo J, Lazar MA and Brown M . (2000). Cell, 103, 843–852.
Smale ST . (1997). Biochem. Biophys. Acta, 1351, 73–88.
Strasser A, O'Connor L and Dixit VM . (2000). Annu. Rev. Biochem., 69, 217–245.
Susin SA, Lorenzo HK, Zamzami N, Marzo I, Snow BE, Brothers GM, Mangion J, Jacotot E, Costantini P, Loeffler M, Larochette N, Goodlett DR, Aebersold R, Siderovski D, Penninger PJM and Kroemer G . (1999). Nature, 397, 441–446.
Szak ST, Mays D and Pietenpol JA . (2001). Mol. Cell. Biol., 21, 3375–3386.
Vogelstein B, Lane D and Levine AJ . (2000). Nature, 408, 307–310.
Wang X . (2001). Genes Dev., 22, 2922–2933.
Wu M, Xu L, Li X, Zhai Z and Shu H . (2002). J. Biol. Chem., 277, 25617–25623.
Wu X, Bayle JH, Olson D and Levine AJ . (1993). Genes Dev., 7, 1126–1132.
Yoshida H, Kong YY, Yoshida R, Elia AJ, Hakem A, Hakem R, Penninger JM and Mak TW . (1998). Cell, 94, 739–750.
Acknowledgements
We thank Bert Vogelstein and Jun Huang for reagents. This work was supported by grants from the National Natural Science Foundation of China (39925016 and 90208016), the Chinese High Technology Program (2003AA221030), and the Special Funds for Major State Basic Research of China (G19990539).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wu, M., Xu, LG., Su, T. et al. AMID is a p53-inducible gene downregulated in tumors. Oncogene 23, 6815–6819 (2004). https://doi.org/10.1038/sj.onc.1207909
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1207909
Keywords
This article is cited by
-
YTHDC1 as a tumor progression suppressor through modulating FSP1-dependent ferroptosis suppression in lung cancer
Cell Death & Differentiation (2023)
-
The microRNA-3622 family at the 8p21 locus exerts oncogenic effects by regulating the p53-downstream gene network in prostate cancer progression
Oncogene (2022)
-
A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers
Nature Communications (2022)
-
ROS-Dependent Lipid Peroxidation and Reliant Antioxidant Ferroptosis-Suppressor-Protein 1 in Rheumatoid Arthritis: a Covert Clue for Potential Therapy
Inflammation (2021)
-
Ferroptosis, radiotherapy, and combination therapeutic strategies
Protein & Cell (2021)