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AMID is a p53-inducible gene downregulated in tumors

Abstract

AMID, also called PRG3, is an AIF-homologous and mitochondria-associated protein that has been implicated in caspase-independent apoptosis. In this report, we demonstrated that human AMID gene promoter was activated by p53 in reporter gene assays. Chromatin immunoprecipitation experiments indicated that p53 could bind to human AMID promoter. Deletion mutagenesis indicated that human AMID promoter contains two p53-responsive elements. Furthermore, expression array analysis indicated that human AMID mRNA expression was downregulated in a majority of human tumors. Our findings point to the possibility that AMID is a p53-downstream gene involved in tumorigenesis.

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Acknowledgements

We thank Bert Vogelstein and Jun Huang for reagents. This work was supported by grants from the National Natural Science Foundation of China (39925016 and 90208016), the Chinese High Technology Program (2003AA221030), and the Special Funds for Major State Basic Research of China (G19990539).

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Correspondence to Hong-Bing Shu.

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Wu, M., Xu, LG., Su, T. et al. AMID is a p53-inducible gene downregulated in tumors. Oncogene 23, 6815–6819 (2004). https://doi.org/10.1038/sj.onc.1207909

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