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Overexpression of the thymosin β-4 gene is associated with increased invasion of SW480 colon carcinoma cells and the distant metastasis of human colorectal carcinoma

Oncogene volume 23, pages 6666–6671 (2004)Cite this article

Abstract

Cell–matrix and cell–cell adhesive interactions play important roles in the normal organization and stabilization of the cell layer in epithelial tissue. Alterations in the expression and function of these adhesion systems that cause a switch to a migratory phenotype in tumor invasion and metastasis are critical for the malignant conversion of epithelial cells. Thymosin β-4 (Tβ-4) is the major actin-sequestering protein that has been shown to be upregulated in a wide variety of human carcinomas and has been implicated to be involved in altering the motility of certain tumors. We have recently demonstrated that the growth rate, colony formation in soft agar, and motility, all good indicators for malignant progression, of SW480 colon carcinoma cells are dramatically increased by enforced Tβ-4 expression. To test the hypothesis that overexpression of this G-actin sequestering peptide also promotes tumor invasion, we examined not only the invasion capability of Tβ-4-overexpressing SW480 cells, but also the expression levels of Tβ-4 as well as several proteins that participate in different stages of tumor progression in matched samples of human primary colorectal adenocarcinoma and liver metastases from several patients. A marked increase on the invasiveness in Tβ-4-overexpressing SW480 cells with increased levels and activity of matrix metalloproteinase-7 (MMP-7) was observed. Furthermore, the levels of Fas as well as the susceptibility to Fas ligand-mediated apoptosis in Tβ-4-overexpressing cells were significantly decreased. Interestingly, the levels of Tβ-4 mRNA, β-catenin, c-Myc, and MMP-7 in metastatic liver lesions were relatively higher, whereas the levels of E-cadherin and Fas were significantly lower than those in the matched primary colorectal tumors. These results suggest that upregulation of Tβ-4, by promoting the disruption of cell–cell adhesion and a consequential activation of the β-catenin signaling, could be a key event in the acquisition of growth advantages as well as invasive phenotypes in human colorectal carcinomas.

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Acknowledgements

This study was supported in part by Grants NSC 90-2314-B-075-80, NSC 91-2320-B-010-096, and NSC 92-2320-B-010-035 from the National Science Council of the Republic of China.

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Authors and Affiliations

  1. Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China

    Wei-Shu Wang

  2. Division of Medical Oncology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China

    Wei-Shu Wang & Po-Min Chen

  3. Institute of Biopharmaceutical Science, College of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China

    Hung-Liang Hsiao & Yeu Su

  4. Division of Colorectal Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China

    Huann-Sheng Wang

  5. Department of Pathology, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China

    Wen-Yih Liang

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Correspondence to Yeu Su.

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Wang, WS., Chen, PM., Hsiao, HL. et al. Overexpression of the thymosin β-4 gene is associated with increased invasion of SW480 colon carcinoma cells and the distant metastasis of human colorectal carcinoma. Oncogene 23, 6666–6671 (2004). https://doi.org/10.1038/sj.onc.1207888

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