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Differential modulation of Myb family genes by Ets-2

Oncogene volume 23, pages 4177–4181 (2004)Cite this article

Abstract

The myb family of genes encodes highly homologous nuclear transcription factors that play distinct roles in the development of breast, germ cells and hematoid organs. While the mechanisms associated with the regulation of these genes remain unknown, the transactivation of c-Myb was previously shown to be upregulated by transcriptional cooperation with Ets-2. The present study examines the transactivation potential of the myb gene family in cooperation with Ets-2. A-Myb and c-Myb showed similar transcriptional cooperation with Ets-2, but not with Ets-1. Interestingly, B-Myb showed no cooperative activity with Ets-2 or Ets-1. Additionally, deletion mutants of A-Myb or c-Myb, where the C-terminal negative regulatory domain was deleted, did not abrogate their ability to cooperate with Ets-2. However, the deletion mutant of B-Myb, where the C-terminal positive regulatory domain was deleted, restored its ability to cooperate with Ets-2. Furthermore, studies using a series of ‘domain-swapped’ mutants between c-Myb and B-Myb revealed that the C-terminus of B-Myb, which is responsible for the protein's transactivation potential, blocks transcriptional cooperation with Ets-2. These results suggest that the myb gene family can be differentially modulated by Ets-2, and that the C-terminus is the domain that regulates this activity.

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Acknowledgements

The pTA-luc plasmid was a generous gift from Drs Scott Ness and Thomas Graf. We thank Dr. Stacey Baker (Fels Institute for Cancer Research, Philadelphia, USA) for the critical reading of the manuscript.

This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (01-PJ3-PG6-01GN07-0004).

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  1. Anthony D Kang

    Present address: Fels Institute for Molecular Biology and Cancer, Temple University, Philadelphia, PA, 19140, USA

Authors and Affiliations

  1. Department of Cellular Medicine and Biology, Cell and Gene Therapy Institute, Catholic University of Korea, 505, Banpo-dong, Seo-cho Ku, Seoul, 137-701, South Korea

    Anthony D Kang & Il-Hoan Oh

  2. Department of Clinical Pathology, Catholic University of Korea, Seoul, South Korea

    Gyeongsin Park

  3. Korea University, Seoul, South Korea

    Yul-Hong Kim

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Correspondence to Il-Hoan Oh.

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Kang, A., Park, G., Kim, YH. et al. Differential modulation of Myb family genes by Ets-2. Oncogene 23, 4177–4181 (2004). https://doi.org/10.1038/sj.onc.1207537

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