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  • Original Paper
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Altered interaction of HDAC5 with GATA-1 during MEL cell differentiation

Oncogene volume 22, pages 9176–9184 (2003)Cite this article

Abstract

The transcription factor GATA-1 plays a significant role in erythroid differentiation and association with CBP stimulates its activity by acetylation. It is possible that histone deacetylases (HDACs) repress the activity of GATA-1. In the present study, we investigated whether class I and class II HDACs interact with GATA-1 to regulate its function and indeed, GATA-1 is directly associated with HDAC3, HDAC4 and HDAC5. The expression profiling and our previous observation that GATA-2 interacts with members of the HDAC family prompted us to investigate further the biological relevance of the interaction between GATA-1 and HDAC5. Coexpression of HDAC5 suppressed the transcriptional potential of GATA-1. Our results demonstrated that GATA-1 and HDAC5 colocalized to the nucleus of murine erythroleukemia (MEL) cells. Furthermore, a portion of HDAC5 moved to the cytoplasm concomitant with MEL cell erythroid differentiation, which was induced by treatment with N,N′-hexamethylenebisacetamide. These observations support the suggestion that control of the HDAC5 nucleocytoplasmic distribution might be associated with MEL cell differentiation, possibly through regulated GATA-1 transactivation.

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References

  • Bertos NR, Wang AH and Yang XJ . (2001). Biochem. Cell Biol., 79, 243–252.

  • Blobel GA, Nakajima T, Eckner R, Montminy M and Orkin SH . (1998). Proc. Natl. Acad. Sci. USA, 95, 2061–2066.

  • Boyes J, Byfield P, Nakatani Y and Ogryzko V . (1998). Nature, 396, 594–598.

  • Briegel K, Lim KC, Plank C, Beug H, Engel JD and Zenke M . (1993). Genes Dev., 7, 1097–1109.

  • Brownell JE, Zhou J, Ranalli T, Kobayashi R, Edmondson DG, Roth SY and Allis CD . (1996). Cell, 84, 843–851.

  • Chen H, Lin RJ, Schiltz RL, Chakravarti D, Nash A, Nagy L, Privalsky ML, Nakatani Y and Evans RM . (1997). Cell, 90, 569–580.

  • Conscience JF, Miller RA, Henry J and Ruddle FH . (1977). Exp. Cell Res., 105, 401–412.

  • Cress WD and Seto E . (2000). J. Cell Physiol., 184, 1–16.

  • Crispino JD, Lodish MB, MacKay JP and Orkin SH . (1999). Mol. Cell, 3, 219–228.

  • Crotta S, Nicolis S, Ronchi A, Ottolenghi S, Ruzzi L, Shimada Y, Migliaccio AR and Migliaccio G . (1990). Nucleic Acids Res., 18, 6863–6869.

  • Emiliani S, Fischle W, Van Lint C, Al-Abed Y and Verdin E . (1998). Proc. Natl. Acad. Sci. USA, 95, 2795–2800.

  • Evans T and Felsenfeld G . (1989). Cell, 58, 877–885.

  • Fujiwara Y, Browne CP, Cunniff K, Goff SC and Orkin SH . (1996). Proc. Natl. Acad. Sci. USA, 93, 12355–12358.

  • Gao L, Cueto MA, Asselbergs F and Atadja P . (2002). J. Biol. Chem., 277, 25748–25755.

  • George KM, Leonard MW, Roth ME, Lieuw KH, Kioussis D, Grosveld F and Engel JD . (1994). Development, 120, 2673–2686.

  • Grozinger CM, Hassig CA and Schreiber SL . (1999). Proc. Natl. Acad. Sci. USA, 96, 4868–4873.

  • Grozinger CM and Schreiber SL . (2000). Proc. Natl. Acad. Sci. USA, 97, 7835–7840.

  • Heberlein C, Fischer KD, Stoffel M, Nowock J, Ford A, Tessmer U and Stocking C . (1992). Mol. Cell. Biol., 12, 1815–1826.

  • Hong L, Schroth GP, Matthews HR, Yau P and Bradbury EM . (1993). J. Biol. Chem., 268, 305–314.

  • Hu E, Chen Z, Fredrickson T, Zhu Y, Kirkpatrick R, Zhang GF, Johanson K, Sung CM, Liu R and Winkler J . (2000). J. Biol. Chem., 275, 15254–15264.

  • Ikonomi P, Rivera CE, Riordan M, Washington G, Schechter AN and Noguchi CT . (2000). Exp. Hematol., 28, 1423–1431.

  • Knoepfler PS and Eisenman RN . (1999). Cell, 99, 447–450.

  • Laverriere AC, MacNeill C, Mueller C, Poelmann RE, Burch JB and Evans T . (1994). J. Biol. Chem., 269, 23177–23184.

  • Lee DY, Hayes JJ, Pruss D and Wolffe AP . (1993). Cell, 72, 73–84.

  • Leonard MW, Lim KC and Engel JD . (1993). Development, 119, 519–531.

  • McKinsey TA, Zhang CL, Lu J and Olson EN . (2000). Nature, 408, 106–111.

  • Mizzen CA, Yang XJ, Kokubo T, Brownell JE, Bannister AJ, Owen-Hughes T, Workman J, Wang L, Berger SL, Kouzarides T, Nakatani Y and Allis CD . (1996). Cell, 87, 1261–1270.

  • Ng HH and Bird A . (2000). Trends Biochem. Sci., 25, 121–126 3_00001551 3_00001551.

  • Norton VG, Imai BS, Yau P and Bradbury EM . (1989). Cell, 57, 449–457.

  • Ogryzko VV, Schiltz RL, Russanova V, Howard BH and Nakatani Y . (1996). Cell, 87, 953–959.

  • Orkin SH . (1992). Blood, 80, 575–581.

  • Orkin SH, Harosi FI and Leder P . (1975). Proc. Natl. Acad. Sci. USA, 72, 98–102.

  • Ozawa Y, Towatari M, Tsuzuki S, Hayakawa F, Maeda T, Miyata Y, Tanimoto M and Saito H . (2001). Blood, 98, 2116–2123.

  • Pazin MJ and Kadonaga JT . (1997). Cell, 89, 325–328.

  • Pevny L, Simon MC, Robertson E, Klein WH, Tsai SF, D’Agati V, Orkin SH and Costantini F . (1991). Nature, 349, 257–260.

  • Shore D . (2000). Proc. Natl. Acad. Sci. USA, 97, 14030–14032.

  • Spencer TE, Jenster G, Burcin MM, Allis CD, Zhou J, Mizzen CA, McKenna NJ, Onate SA, Tsai SY, Tsai MJ and O'Malley BW . (1997). Nature, 389, 194–198.

  • Takahashi T, Suwabe N, Dai P, Yamamoto M, Ishii S and Nakano T . (2000). Oncogene, 19, 134–140.

  • Taunton J, Hassig CA and Schreiber SL . (1996). Science, 272, 408–411.

  • Tong JK, Hassig CA, Schnitzler GR, Kingston RE and Schreiber SL . (1998). Nature, 395, 917–921.

  • Towatari M, May GE, Marais R, Perkins GR, Marshall CJ, Cowley S and Enver T . (1995). J. Biol. Chem., 270, 4101–4107.

  • Tsai FY, Keller G, Kuo FC, Weiss M, Chen J, Rosenblatt M, Alt FW and Orkin SH . (1994). Nature, 371, 221–226.

  • Tsai FY and Orkin SH . (1997). Blood, 89, 3636–3643.

  • Tsang AP, Visvader JE, Turner CA, Fujiwara Y, Yu C, Weiss MJ, Crossley M and Orkin SH . (1997). Cell, 90, 109–119.

  • Tsuzuki S, Towatari M, Saito H and Enver T . (2000). Mol. Cell. Biol., 20, 6276–6286.

  • Vettese-Dadey M, Grant PA, Hebbes TR, Crane-Robinson C, Allis CD and Workman JL . (1996). EMBO J., 15, 2508–2518.

  • Wade PA . (2001). Hum. Mol. Genet., 10, 693–698.

  • Wang AH, Bertos NR, Vezmar M, Pelletier N, Crosato M, Heng HH, Th'ng J, Han J and Yang XJ . (1999). Mol. Cell Biol., 19, 7816–7827.

  • Weiss MJ, Keller G and Orkin SH . (1994). Genes Dev., 8, 1184–1197.

  • Weiss MJ and Orkin SH . (1995). Exp. Hematol., 23, 99–107.

  • Weiss MJ, Yu C and Orkin SH . (1997). Mol. Cell. Biol., 17, 1642–1651.

  • Yamamoto M, Ko LJ, Leonard MW, Beug H, Orkin SH and Engel JD . (1990). Genes Dev., 4, 1650–1662.

  • Yamamoto T and Horikoshi M . (1997). J. Biol. Chem., 272, 30595–30598.

  • Yang WM, Inouye C, Zeng Y, Bearss D and Seto E . (1996a). Proc. Natl. Acad. Sci. USA, 93, 12845–12850.

  • Yang WM, Yao YL, Sun JM, Davie JR and Seto E . (1997). J. Biol. Chem., 272, 28001–28007.

  • Yang XJ, Ogryzko VV, Nishikawa J, Howard BH and Nakatani Y . (1996b). Nature, 382, 319–324.

  • Yomogida K, Ohtani H, Harigae H, Ito E, Nishimune Y, Engel JD and Yamamoto M . (1994). Development, 120, 1759–1766.

  • Zhang P, Zhang X, Iwama A, Yu C, Smith KA, Mueller BU, Narravula S, Torbett BE, Orkin SH and Tenen DG . (2000). Blood, 96, 2641–2648.

  • Zhang Y, LeRoy G, Seelig HP, Lane WS and Reinberg D . (1998). Cell, 95, 279–289.

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Acknowledgements

We are grateful to S Suzuki, M Isomura, and C Wakamatsu for technical assistance.

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Authors and Affiliations

  1. Department of Molecular Medicine, Graduate School of Medicine, Nagoya University, Nagoya, 466-8550, Japan

    Kouichi Watamoto, Masayuki Towatari, Yukiyasu Ozawa, Yasuhiko Miyata, Mitsunori Okamoto, Akihiro Abe & Tomoki Naoe

  2. National Nagoya Hospital, Nagoya, 460-0001, Japan

    Hidehiko Saito

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  1. Kouichi Watamoto
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Correspondence to Masayuki Towatari.

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Watamoto, K., Towatari, M., Ozawa, Y. et al. Altered interaction of HDAC5 with GATA-1 during MEL cell differentiation. Oncogene 22, 9176–9184 (2003). https://doi.org/10.1038/sj.onc.1206902

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