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Involvement of aquaporins in colorectal carcinogenesis

Oncogene volume 22, pages 6699–6703 (2003)Cite this article

Abstract

Aquaporins (AQPs) are important in controlling water permeability. As AQP1 is known as a serum-responsive gene, we hypothesized that AQP expression may be involved in the development of human cancer. By reverse transcriptase–polymerase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and colorectal cancer cell lines. Western blot analysis confirmed their expression in four of these cell lines. In situ hybridization demonstrated that during colorectal carcinogenesis, the expression of AQPs 1 and 5 was induced in early-stage disease (early dysplasia) and maintained through the late stages of colon cancer development. Expression of AQPs 1 and 5 was maintained even in metastatic lesions in the liver. These findings demonstrate that the expression of several AQPs is found in tumor cells and is associated with an early stage of colorectal cancer development. These novel observations suggest that multiple AQP expression may be advantageous to tumorigenesis, which may lead to a better understanding of colorectal carcinogenesis.

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Acknowledgements

We thank Dr Li K Su, who provided total RNA for the three colon cell lines used in this study. The study was supported in part by NIH/NCI Grant P50 CA96784-01 (to CM), American Cancer Society Grant RPG-98-054 (to LM), Fondation de France, AP-HP and Lilly Fondation Grant (to J-CS), Cancer Center Grant P30 CA 16620 (to MD Anderson Cancer Center), and Tobacco Research Fund from State of Texas (to MD Anderson Cancer Center).

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Authors and Affiliations

  1. The Head and Neck Cancer Research Division, Department of Otolaryngology, The Johns Hopkins University School of Medicine, Baltimore, 21205, MD, USA

    Chulso Moon, Juna Lee, Mohammad Obaidul Hoque, Barry Trink & David Sidransky

  2. Department of Oncology and Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, 21205, MD, USA

    Chulso Moon & David Sidransky

  3. Division of Cancer Medicine, Gustave Roussy Institute, 39 Rue Camille Desmoulins, Villejuif, 94805, France

    Jean-Charles Soria

  4. Department of Thoracic/Head and Neck Medical Oncology, Molecular Biology Laboratory, The University of Texas MD Anderson Cancer Center, Houston, 77030, TX, USA

    Se Jin Jang, Yoon Soo Chang & Li Mao

  5. Pathology Department, Hospital Tenon, AP-HP, 4 rue de la Chine, Paris, 75020, France

    Mathilde Sibony

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  1. Chulso Moon
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  2. Jean-Charles Soria
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Correspondence to Chulso Moon.

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Moon, C., Soria, JC., Jang, S. et al. Involvement of aquaporins in colorectal carcinogenesis. Oncogene 22, 6699–6703 (2003). https://doi.org/10.1038/sj.onc.1206762

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