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fau and its ubiquitin-like domain (FUBI) transforms human osteogenic sarcoma (HOS) cells to anchorage-independence

Oncogene volume 22, pages 1817–1821 (2003)Cite this article

Abstract

Arsenite is the most likely carcinogenic form of arsenic in the environment. Previously, expression cloning for cDNAs whose overexpression confers arsenite-resistance in Chinese hamster V79 cells identified two genes: fau and a novel gene, asr2. The fau gene encodes a ubiquitin-like protein (here called FUBI) fused to the ribosomal S30 protein. Since the expression of the fox sequence (antisense to fau) increased the tumorigenicity of a mouse sarcoma virus, it was proposed that fau might be a tumor suppressor gene. We intended to test its ability to block arsenite-induced transformation of human osteogenic sarcoma (HOS) cells to anchorage-independence. Instead, we found that overexpressing fau itself was able to transform HOS cells. When the two domains were expressed separately, only FUBI was transforming and only the S30 domain conferred arsenite resistance. An incidental finding was the transforming activity of the selectable marker, hyg. FUBI belongs to the ubiquitin-like protein group that is capable of forming conjugates to other proteins, although none have so far been identified. Alternatively, FUBI may act as a substitute or inhibitor of ubiquitin, to which it is most closely related, or to close ubiquitin-like relatives UCRP, FAT10, and/or Nedd8.

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References

  • Baker RT, Williamson NA and Wettenhall REH . (1996). J. Biol. Chem., 271, 13549–13555.

  • Chen R-H, Waters JC, Salmon ED and Murray AW . (1996). Science, 274, 242–246.

  • D'Cunha J, Knight E, Haas AL, Truitt RL and Borden EC . (1996). Proc. Natl. Acad. Sci. USA, 93, 211–215.

  • Finkel MP, Reilly Jr CA and Biskis BO . (1976). Recent Results Cancer Res, 54, 92–103.

  • Finley D, Bartel B and Varshavsky A . (1989). Nature, 338, 394–401.

  • Hermeking H, Lengauer C, Polyak K, He TC, Zhang L, Thiagalingam S, Kinzler KW and Vogelstein B . (1997). Mol. Cell, 1, 3–11.

  • Hiemstra PS, van den Barselaar MT, Roest M, Nibbering PH and van Furth R . (1999). J. Leukocyte Biol, 66, 423–428.

  • Hochstrasser M . (1996). Cell, 84, 813–815.

  • Hochstrasser M . (2000). Science, 289, 563–564.

  • Hori T, Osaka F, Chiba T, Miyamoto C, Okabayashi K, Shimbara N, Kato S and Tanaka K . (1999). Oncogene, 18, 6829–6834.

  • Huang S-C and Lee T-C . (1998). Carcinogenesis, 19, 889–896.

  • Huang C, Li J, Din M, Wang L, Shi X, Castranova V, Vallyathan V, Ju G and Costa M . (2001). Mol. Cell. Biochem., 222, 29–34.

  • Hummer BT, Li X-L and Hassel BA . (2001). J. Virol., 75, 7774–7777.

  • Jentsch S and Pyrowolakis G . (2000). Cell Biol, 10, 335–342.

  • Johnson DG . (2000). Mol. Carcinogenesis, 27, 151–157.

  • Klemperer NS and Pickart CM . (1989). J. Biol. Chem., 264, 19245–19252.

  • Kurz T, Pintard L, Willis JH, Hamill DR, Gonczy P, Peter M and Bowerman B . (2002). Science, 295, 1294–1298.

  • Li Y and Benezra R . (1996). Science, 274, 246–248.

  • Lin X and Costa M . (1994). Environ. Health Perspect., 102, 289–292.

  • Liu Y-C, Pan J, Zhang C, Fan W, Collinge M, Bender JR and Weissman SM . (1999). Proc. Natl. Acad. Sci. USA, 96, 4313–4318.

  • Loeb KR and Haas AL . (1992). J. Biol. Chem., 267, 7806–7813.

  • McLaughlin PMJ, Helfrich W, Kok K, Mulder M, Hu SW, Brinker MGL, Ruiters MHJ, de Leij LFMH and Buys CHCM . (2000). Int. J. Cancer, 85, 871–876.

  • Michiels L, Van der Rauwelaert E, Van Hasselt F, Kas K and Merregaert J . (1993). Oncogene, 8, 2537–2546.

  • Miller AC, Blakely WF, Livengood D, Whittaker T, Xu J, Ejnik JW, Hamilton MM, Parlette E, St John T, Gerstenberg HM and Hsu H . (1998). Environ. Health Perspect., 106, 465–471.

  • Nakamura M and Tanigawa Y . (1999). J. Biol. Chem., 274, 18026–18032.

  • Nakamura M, Xavier RM and Tanigawa Y (1996). J. Immunol., 156, 532–538.

  • Nakamura M, Xavier RM, Tsunematsu T and Tanigawa Y . (1995). Proc. Natl. Acad. Sci. USA, 92, 3463–3467.

  • Podust VN, Brownell JE, Gladysheva TB, Luo RS, Wang C, Coggins MB, Pierce JW, Lightcap ES and Chau V . (2000). Proc. Natl. Acad. Sci. USA, 97, 4579–4584.

  • Polyak K, Xia Y, Zweier JL, Kinzler KW and Vogelstein B . (1997). Nature, 18, 300–305.

  • Potter JL, Narasimhan J, Mende-Mueller L and Haas AL . (1999). J. Biol. Chem., 274, 25061–25068.

  • Raasi S, Schmidtke G and Groettrup M . (2001). J. Biol. Chem., 276, 35334–35343.

  • Ramirez P, Eastmond DA, Laclette JP and Ostrosky-Wegman P . (1997). Mutat. Res., 386, 291–298.

  • Rani AS and Kumar S . (1992). Carcinogenesis, 13, 2021–2027.

  • Read MA, Brownell JE, Gladysheva TB, Hottelet M, Parent LA, Coggins MB, Pierce JW, Produst VN, Luo RS, Chau V and Palombella VJ . (2000). Mol. Cell. Biol., 20, 2326–2333.

  • Redman KL and Burris GW . (1996). Biochem. J., 315, 21.

  • Rhim JS, Park DK, Arnstein P, Huebner RJ, Weisburger EK and Nelson-Rees WA . (1975). Nature, 256, 751–753.

  • Rossman TG, Visalli MA, Uddin AN and Hu Y . (2001). Arsenic Exposure and Health Effects. Chappel WR, Abernathy CO and Calderon R. (eds), Elsevier Science B.V.: Amsterdam, Chapter 27, pp. 285–295.

    Google Scholar 

  • Rossman TG and Wang Z . (1999). Carcinogenesis, 20, 311–316.

  • Sambrook J, Fritsch EF and Maniatis T . (1989). Molecular Cloning. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.

    Google Scholar 

  • Shin S-I, Freedman VH, Risser R and Pollack R . (1975). Proc. Natl. Acad. Sci. USA, 72, 4435–4439.

  • Tateishi K, Omata M, Tanaka K and Chiba T . (2001). J. Cell Biol., 155, 571–579.

  • Tornaletti S and Hanawalt PC . (1999). Biochimie, 81, 139–146.

  • Vogt B and Rossman TG . (2001). Mutat. Res., 478, 59–168.

  • Wang Z and Rossman TG . ( 1994). Bio. Tech., 16, 460–463.

  • Wright G, Albert A and Mobashery S . (1998). Aminoglycoside Antibiotics. New York: Kluwer Academic/Plenum Publishers, pp. 27–69.

    Google Scholar 

  • Wong JM, Mafune K, Yow H, Rivers EN, Ravikumar TS, Steel Jr GD and Chen LB . (1993). Cancer Res., 15, 1916–1920.

  • Yang J-J, Kang J-S and Krauss RS . (1998). Mol. Cell. Biol., 18, 2586–2595.

  • Yeh ETH, Gong L and Kamitani T . (2000). Gene, 248, 1–14.

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Acknowledgements

We thank Eleanor Cordisco for her expert help in document preparation. This work was supported by United States Public Health Service Grants R01 ES09252 and P42 ES10344, and is part of NYU's Nelson Institute of Environmental Medicine Center programs supported by Grants ES00260 from NIEHS and CA16087 from NCI.

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Authors and Affiliations

  1. The Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, 10987, NY, USA

    Toby G Rossman, Melissa A Visalli & Elena V Komissarova

  2. The NYU Cancer Institute, New York University School of Medicine, 57 Old Forge Road, Tuxedo, 10987, NY, USA

    Toby G Rossman

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  1. Toby G Rossman
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  2. Melissa A Visalli
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Correspondence to Toby G Rossman.

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Rossman, T., Visalli, M. & Komissarova, E. fau and its ubiquitin-like domain (FUBI) transforms human osteogenic sarcoma (HOS) cells to anchorage-independence. Oncogene 22, 1817–1821 (2003). https://doi.org/10.1038/sj.onc.1206283

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