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  • Oncogenomics
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Patterns of expression of chromosome 17 genes in primary cultures of normal ovarian surface epithelia and epithelial ovarian cancer cell lines

Oncogene volume 22, pages 1568–1579 (2003)Cite this article

Abstract

Oligonucleotide microarray analysis was applied to assess the expression profile of 332 probe sets representing 308 genes or expressed sequence tags (ESTs) that map to chromosome 17 in order to address epigenetic events that result in alterations in gene expression in epithelial ovarian cancer (EOC). Expression profiles were generated from 12 primary cultures derived from normal ovarian surface epithelium (NOSE) and four long-term cultures (TOV-81D, TOV-112D, TOV-21G and OV-90) derived from EOCs that have been previously characterized and shown to mimic features of the tumoral cells from which they were derived. The expression values of all 332 probe sets is highly correlated across the 12 NOSEs (89% correlation coefficients >0.90). In two-way comparisons, differential patterns of gene expression were observed for 157 probe sets for which the expression value of at least one EOC cell line fell outside the limits of the range of expression of the 12 NOSEs. When compared to NOSEs, four genes displayed similar differential patterns of gene expression across all four EOC cell lines, and 26 genes displayed similar differential patterns of gene expression across the three EOC cell lines (TOV-112D, TOV-21G and OV-90) representing tumoral cells derived from the most aggressive disease. A total of 17 genes displayed differential patterns of gene expression greater than threefold in at least one EOC cell line in comparison to NOSE, and three genes were differentially expressed greater than threefold across all aggressive cell lines. The analysis of a selected number of genes by RT–PCR revealed patterns of gene expression comparable to those observed by microarray analysis in the majority of samples tested. Comparison of expression profiles of differentially expressed genes identified by microarray analysis in two-way comparisons of the EOC cell lines and the NOSEs with published reports revealed 10 genes previously implicated in ovarian tumorigenesis and 18 in tumorigenesis of other types of cancer. The differential pattern of gene expression of genes that map to both the p and q arm of chromosome 17 is consistent with the hypothesis that a number of genes that map to this chromosome are implicated in the etiology of ovarian cancer.

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Acknowledgements

We thank L Portelance and M Ladurantaye for their technical assistance. We are grateful to Dr P Drouin, P Gauthier and J Dubuc-Lissoir for the samples. We also thank Emily N Manderson for her useful discussions. This research was supported by grants from the Canadian Institute of Health Research (CIHR) to PNT, A-MM-M and DP, and to A-MM-M PNT, TJH and DP. NP is a recipient of postdoctoral fellowships from La Ligue Nationale Centre le Cancer (France), Programme de bourse d'Excellence du Ministère de 1'Education du Québec (Canada), and the Brigadier-General Herbert Stanley Birkett Memorial Award from the McGill Institutes of Health Research (McGill University Health Centre). DP is a recipient of a Chercheur–Cliniciens Senior from the Fonds de recherche en Santé du Québec (FRSQ) and A-MM-M is a recipient of a Chercheur National fellowship from the FRSQ. PNT is a recipient of the Fraser, Monat, and MacPherson Scholarship (McGill University) and the Stewart Fellowship in Research/Clinical Haematology and Oncology (McGill University Health Centre).

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Authors and Affiliations

  1. Research Institute of the McGill University Health Centre, Montréal, H3G 1A4, Quebéc, Canada

    Nadège Presneau & Patricia N Tonin

  2. Department of Human Genetics, McGill University, Montréal, H3A 1B1, Québec, Canada

    Nadège Presneau, Thomas J Hudson & Patricia N Tonin

  3. Centre de Recherche du Centre Hospitalier de l'Université de Montréal (Hôpital Notre-Dame), Institut du cancer de Montréal, Montréal, H2L 4M1, Québec, Canada

    Anne-Marie Mes-Masson & Diane Provencher

  4. Département de médecine, Université de Montréal, Montréal, H3C 3J7, Québec, Canada

    Anne-Marie Mes-Masson

  5. Montreal Genome Center, McGill University Health Center, Montréal, H3G 1A4, Québec, Canada

    Bing Ge & Thomas J Hudson

  6. Département d'obstétrique-gynécologie Université de Montréal, Montréal, H3C 3J7, Québec, Canada

    Diane Provencher

  7. Department of Medicine, McGill University, Montreal, H3G 1Y6, Québec, Canada

    Thomas J Hudson & Patricia N Tonin

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  1. Nadège Presneau
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Correspondence to Patricia N Tonin.

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Presneau, N., Mes-Masson, AM., Ge, B. et al. Patterns of expression of chromosome 17 genes in primary cultures of normal ovarian surface epithelia and epithelial ovarian cancer cell lines. Oncogene 22, 1568–1579 (2003). https://doi.org/10.1038/sj.onc.1206219

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