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Inhibition of growth of mouse gastric cancer cells by Runx3, a novel tumor suppressor

Oncogene volume 21, pages 8351–8355 (2002)Cite this article

Abstract

We reported recently that the silencing of RUNX3 is causally related to gastric cancer in humans. Here we report that in three of four cell lines derived from N-methyl-N-nitrosourea-induced mouse glandular stomach carcinomas, Runx3 is silenced due to hypermethylation of CpG islands in the promoter region, as we also observed for human gastric cancer cells. Although two of the sites we tested in the promoter of the fourth line were not methylated, in all four cases the silencing of Runx3 could be reversed by treatment of the cells with 5′-azacytidine and trichostatin A. Interestingly, the exogenous expression of RUNX3 in cell lines that do not express the endogenous gene caused an inhibition of growth in soft agar, suggesting that anchorage-independent growth could be used as an assay of RUNX3 activity in vitro. These observations suggest that the mouse system described here may be useful as a model for the study of human gastric carcinogenesis.

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Acknowledgements

We thank Motomi Osato for advice on the in vivo tumorigenicity assay.

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Author notes

  1. Yoshiaki Ito

    Present address: Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, 117609, Singapore

  2. Lin-Feng Chen

    Present address: Department of Medicine, Gladstone Institute of Virology and Immunology, University of California, San Francisco, 94141, California, CA, USA

Authors and Affiliations

  1. Department of Viral Oncology, Institute for Virus Research, Kyoto University, Shogoin, 606-8507, Sakyo-ku Kyoto, Japan

    Wei-Hui Guo, Wei-Hui Guo, Li-Qin Weng, Li-Qin Weng, Kosei Ito, Kosei Ito, Lin-Feng Chen & Yoshiaki Ito

  2. Laboratory of Pathology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, 464-8681, Nagoya, Japan

    Hayao Nakanishi & Masae Tatematsu

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Correspondence to Yoshiaki Ito.

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Guo, WH., Weng, LQ., Ito, K. et al. Inhibition of growth of mouse gastric cancer cells by Runx3, a novel tumor suppressor. Oncogene 21, 8351–8355 (2002). https://doi.org/10.1038/sj.onc.1206037

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