Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Oncogenomics
  • Published:

Novel candidate tumor marker genes for lung adenocarcinoma

Oncogene volume 21, pages 7598–7604 (2002)Cite this article

Abstract

Using the representational difference analysis (RDA) technique on pooled mRNA of five primary lung adenocarcinomas and their corresponding non-neoplastic lung tissues, we identified six genes that were putatively overexpressed in this type of lung cancer. Five corresponded to previously isolated genes, while one (Lc19) matched with the sequence of an unannotated EST. Real-time RT–PCR analyses of expression levels in a panel of 34 paired primary non-small cell lung cancer (NSCLC) and corresponding grossly normal appearing lung tissues confirmed the common overexpression of these genes in non-small cell lung cancer. Among these genes, overexpression of Lc19, hyaluronan binding protein 2 (HABP2) and crystalline-mu appeared more specific to adenocarcinoma, whereas ceruloplasmin, integrin α-11 and collagen type XI alpha 1 were overexpressed at high frequency among both adenocarcinoma and squamous cell carcinoma. These genes represent novel candidate tumor biomarker genes for NSCLC and its histological subtypes.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2

Similar content being viewed by others

References

  • Bhattacharjee A, Richards WG, Staunton J, Li C, Monti S, Vasa P, Ladd C, Behesti J, Bueno R, Gillette M, Loda M, Weber G, Mark EJ, Lander ES, Wong W, Johnson BE, Gobub TR, Sugarbaker DJ, Meyerson M . 2001 Proc. Natl. Acad. Sci. USA 98: 13790–13795

  • Braun BS, Frieden R, Lessnick S, May WA, Denny CT . 1995 Mol. Cell. Biol. 15: 4623–4630

  • Chen H, Phillips HA, Callen DF, Kim RY, Wistow GJ, Antonarakis SE . 1992 Genomics 14: 1115–1116

  • Choi-Miura N-H, Tobe T, Sumiya J, Nakano Y, Sano Y, Mazda T, Tomita M . 1996 J. Biochem. 119: 1157–1165

  • Garber ME, Troyanskaya OG, Schluens K, Petersen S, Thaesler Z, Pacyna-Gengelbach M, van de Rijn M, Rosen GD, Perou CM, Whyte RI, Altman RB, Brown PO, Botstein D, Petersen I . 2001 Proc. Natl. Acad. Sci. USA 98: 13784–13789

  • Greenlee RT, Hill-Harmon MB, Murray T, Thun M . 2001 CA Cancer J. Clin. 51: 15–36

  • Griffith AJ, Sprunger LK, Sirko-Osadsa DA, Tiller GE, Meisler MH, Warman ML . 1998 Am. J. Hum. Genet. 62: 816–823

  • Hsu N-Y, Ho H-C, Chow K-C, Lin T-Y, Shih C-S, Wang L-S, Tsai C-M . 2001 Cancer Res. 61: 2727–2731

  • Hibi K, Liu Q, Beaudry GA, Madden SL, Westra WH, Wehage SL, Yang SC, Heitmiller RF, Bertelsen AH, Sidransky D, Jen J . 1998 Cancer Res. 58: 5690–5694

  • Hirota M, Fukushima K, Terasaki PI, Terashita GY, Galton J, Kawahara M . 1985 Cancer Res. 45: 6453–6456

  • Hubank M, Schatz DG . 1994 Nucleic Acid Res. 22: 5640–5648

  • Kersting M, Friedl C, Kraus A, Behn M, Pankow W, Schuermann M . 2000 J. Clin. Oncol. 18: 3221–3229

  • Iwao K, Watanabe T, Fujiwara Y, Takami K, Kodama K, Higashiyama M, Yokouchi H, Ozaki K, Monden M, Tanigami A . 2001 Int. J. Cancer 91: 433–437

  • Lehnert K, Ni J, Leung E, Bough SM, Weaver A, Yao W-P, Liu D, Wang S-X, Morris CM, Krissansen GW . 1999 Genomics 60: 179–187

  • Liloglou T, Maloney P, Xinarianos G, Hulbert M, Walshaw MJ, Gosney JR, Turnbull L, Field JK . 2001 Cancer Res. 61: 1624–1628

  • Linder MC, Moor JR, Wright K . 1981 J. Natl. Cancer Inst. 67: 263–275

  • Lisitsyn N, Lisitsyn N, Wigler M . 1993 Science 259: 946–951

  • Lubin R, Zalcman LR, Bouchet L, Tredanel J, Legros Y, Cazals D, Hirsch A, Soussi T . 1995 Nat. Med. 1: 701–702

  • Mao L, Hruban RH, Tockman MS, Erozan YS, Askin F, Sidransky D . 1994 Cancer Res. 54: 1634–1637

  • Manda R, Kohno T, Matsuno Y, Takenoshita S, Kuwano H, Yokota J . 1999 Genomics 61: 5–14

  • Morris NP, Bachinger HP . 1987 J. Biol. Chem. 262: 11345–11350

  • Mountain CF . 1997 Chest 111: 1710–1717

  • Nah HD, Barembaum M, Upholt WB . 1992 J. Biol. Chem. 267: 22581–22586

  • Palmisano WA, Divine KK, Saccomanno G, Gilliland FD, Baylin SB, Herman JG, Belinsky SA . 2000 Cancer Res. 60: 5954–5958

  • Pastorian K, Hawel L, Byus CV . 2000 Analytical Biochem. 283: 89–98

  • Richards AJ, Yates JRW, Williams R, Payne SJ, Pope FM, Scott JD, Snead MP . 1996 Hum. Mol. Genet. 5: 1339–1343

  • Rom WN, Hay JG, Lee TC, Jiang Y, Tchou-Wong K-M . 2000 Am. J. Respir. Crit. Care Med. 161: 1355–1367

  • Schraml P, Shipman R, Colombi M, Ludwig CU . 1994 Cancer Res. 54: 5236–5240

  • Sumiya J, Asakawa S, Tobe T, Hashimoto K, Saguchi K, Choi-Miura N-H, Shimizu Y, Minoshima S, Shimizu N, Tomita M . 1997 J. Biochem. 122: 983–990

  • Suzzi G, Musso K, Ratcliffe C, Goldstraw P, Pierotti MA, Pastorino U . 1999 Clin. Cancer Res. 5: 2689–2692

  • Thun MJ, Lally CA, Flannery JT, Calle EE, Flanders WD, Heath Jr CW . 1997 J. Natl. Cancer Inst. 89: 1580–1586

  • Tiger C-F, Fougerousse F, Grudnstrom G, Velling T, Gullberg D . 2001 Dev. Biol. 237: 116–129

  • Tockman MS, Gupta PK, Myers JD, Frost JK, Baylin SB, Gold EB, Chase AM, Wilkinson PH, Mulshine JL . 1988 J. Clin. Oncol. 6: 1685–1693

  • Velling T, Kusche-Gullberg M, Sejersen T, Gullberg D . 1999 J. Biol. Chem. 274: 25735–25742

  • Wang T, Hopkins D, Schmidt C, Silva S, Houghton R, Takita H, Repasky E, Reed SG . 2000 Oncogene 19: 1519–1528

  • Welford SM, Gregg J, Chen E, Garrison D, Sorensen PH, Denny CT, Nelson SF . 1998 Nucleic Acid Res. 26: 3059–3065

  • Wigle DA, Jurisica I, Radulovich N, Pintilie M, Rossant J, Liu N, Lu C, Woodgett J, Seiden I, Johnston M, Keshavjee S, Darling G, Winton T, Breitkreutz B-J, Jorgenson P, Tyers M, Shepherd FA, Tsao M-S . 2002 Cancer Res. 62: 3005–3008

  • Wynder EL, Hoffmann D . 1997 Cancer Res. 54: 5284–5295

Download references

Acknowledgements

This work was supported by the Princess Margaret Hospital Foundation through its Clinical Impact Team program. Dr FA Shepherd is the Clive Taylor Chair in Lung Cancer Research at the University Health Network and University of Toronto.

Author information

Author notes

  1. Kan-kan Wang and Ni Liu: K-k Wang and N Liu contributed equally to this study

Authors and Affiliations

  1. University Health Network – Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Ontario, Canada

    Kan-kan Wang, Ni Liu, Nikolina Radulovich, Frances A Shepherd & Mark D Minden

  2. Department of Surgery, University of Toronto, Toronto, M5G 2M9, Ontario, Canada

    Dennis A Wigle & Michael R Johnston

  3. Department of Medicine, University of Toronto, Toronto, M5G 2M9, Ontario, Canada

    Frances A Shepherd & Mark D Minden

  4. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5G 2M9, Ontario, Canada

    Ming-Sound Tsao

Authors
  1. Kan-kan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ni Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nikolina Radulovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dennis A Wigle
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Michael R Johnston
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Frances A Shepherd
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mark D Minden
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ming-Sound Tsao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ming-Sound Tsao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Kk., Liu, N., Radulovich, N. et al. Novel candidate tumor marker genes for lung adenocarcinoma. Oncogene 21, 7598–7604 (2002). https://doi.org/10.1038/sj.onc.1205953

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1205953

Keywords

This article is cited by

Search

Advanced search

Quick links