Abstract
It has recently been suggested that people of the Indian population who carried the codon 149 polymorphism (GAT→GGT) of P21Waf1/Cip1 gene were more susceptible to esophageal cancer and oral cancer than the individuals without that polymorphism. Since esophageal cancer is a high incident neoplasm in China, we analysed the same codon of P21Waf1/Cip1 in the Chinese population. Blood samples from 80 esophageal cancer patients and 80 normal blood donors were collected for DNA extraction. Methods of Polymerase Chain Reaction (PCR) and direct sequencing were used for detection of the polymorphism in codon 149 of P21Waf1/Cip1. Bioinformatics analysis was also thoroughly performed for this gene. No polymorphism was found in all samples tested. Bioinformatics analysis revealed that the so-called polymorphism of codon 149 reported previously was a wrong one. In conclusion, no polymorphism exists in codon 149 of P21Waf1/Cip1. It is not appropriate to use it as a susceptible site of the gene in cancer study.
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Abbreviations
- CDKI:
-
cyclin-dependent kinase inhibitor
- CDS:
-
coding regions
- SNPs:
-
single nucleotide polymorphisms
- UTR:
-
untranslated regions
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Acknowledgements
We gratefully acknowledge Dr YC Chen, Dr JJ Lv, Dr XJ Jin, Dr YP Wang, Dr XJ Sun, HJ Teng for discussion and advice, Miss GB Shi, W Liu, YY Xi, Mrs GR Lu, and Mr T Ning for sequencing and other helps. This work was supported by grants of the National Nature Science Foundation, Human Disease Gene Center of Peking University and Key Research Project Office of Beijing Municipal Science and Technology Commission.
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Xi, YG., Ding, KY., Ren, YH. et al. Esophageal cancer in Chinese population: no polymorphism in codon 149 of P21Waf1/Cip1 cyclin dependent kinase gene. Oncogene 21, 7745–7748 (2002). https://doi.org/10.1038/sj.onc.1205873
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DOI: https://doi.org/10.1038/sj.onc.1205873