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  • Original Paper
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Functional interaction between human papillomavirus type 18 E2 and poly(ADP-ribose) polymerase 1

Abstract

Human papillomavirus E2 protein is a transcription factor of viral gene expression and DNA replication. Here we show that PARP is a positive regulator of the E2 protein of human papillomavirus type 18 (HPV-18). PARP interacted with the COOH terminal region of HPV-18 E2 in vitro. The E2 interaction domain within PARP is located in the NH2-terminal zinc finger motif and the BRCT motif included in the automodification domain. Overexpression of either wild type or the NH2-terminal region of PARP containing zinc finger and BRCT stimulated E2-dependent transcription. Gel retardation assay indicates that PARP augments DNA binding activity of E2 in vitro. We also show that PARP-1 is recruited to E2-dependent promoter in vivo using ChIP assay. These results suggest that PARP serves a transcriptional co-activator in E2-dependent transcription by interacting directly with the HPV E2 protein.

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References

  • Anderson MG, Scoggin KE, Simbulan-Rosenthal CM, Steadman JA . 2000 J. Virol. 74: 2169–2177

  • Bernard BA, Bailly C, Lenoir MC, Darmon M, Thierry F, Yaniv M . 1989 J. Virol. 63: 4317–4324

  • Bork P, Hofmann K, Bucher P, Neuwald AF, Altschul SF, Koonin EV . 1997 FASEB J. 11: 68–76

  • Bouvard V, Storey A, Pim D, Banks L . 1994 EMBO J. 13: 5451–5459

  • Breiding DE, Sverdrup F, Grossel MJ, Moscufo N, Boonchai W, Androphy EJ . 1997 Mol. Cell. Biol. 17: 7208–7219

  • Butler AJ, Ordahl CP . 1999 Mol. Cell. Biol. 19: 296–306

  • Cervellera MN, Sala A . 2000 J. Biol. Chem. 275: 10692–10696

  • Choe J, Vaillancourt P, Stenlund A, Botchan M . 1989 J. Virol. 63: 1743–1755

  • Cripe TP, Haugen TH, Turk JP, Tabatabai F, Schmid IIIPG, Durst M, Gissmann L, Roman A, Turek LP . 1987 EMBO J. 6: 3745–3753

  • d'Adda di Fagagna F, Hande MP, Tong WM, Landorp PM, Wang ZQ, Jackson SP . 1999 Nat. Genet. 23: 76–80

  • D'Amours D, Desnoyers S, D'silva I, Poirier GG . 1999 Biochem. J. 342: 249–268

  • Dantzer F, de La Rubia G, Menissier-de Murcia J, Hostomsky Z, de Murcia G, Schreiber V . 2000 Biochemistry 39: 7559–7569

  • Demeret C, Yaniv M, Thierry F . 1994 J. Virol. 68: 7075–7082

  • de Murcia G, Menissier-de Murcia J . 1994 Trends Biochem. Sci. 19: 172–176

  • Desaintes C, Demeret C, Goyat S, Yaniv M, Thierry F . 1997 EMBO J. 16: 504–514

  • Desaintes C, Goyat S, Garbay S, Yaniv M, Thierry F . 1999 Oncogene 18: 4538–4545

  • Ferro AM, Olivera BM . 1984 J. Biol. Chem. 259: 547–554

  • Funk JO, Waga S, Harry JB, Espling E, Stillman B, Galloway DA . 1997 Genes Dev. 11: 2090–2100

  • Graham FL, Smiley J, Russell WC, Nairn R . 1977 J. Gen. Virol. 36: 59–74

  • Hayaishi O, Ueda K . 1977 Annu. Rev. Biochem. 46: 95–116

  • Huletsky A, Niedergang C, Frechette A, Aubin R, Gaudreau A, Poirier GG . 1985 Eur. J. Biochem. 146: 277–285

  • Jones DL, Thompson DA, Suh-Burgmann E, Grace M, Munger K . 1999 Virology 258: 406–414

  • Kannan P, Wu Y, Wankhade S, Tainsky MA . 1999 Nucleic Acids Res. 27: 866–874

  • Kim JW, Kim K, Kang K, Joe CO . 2000 J. Biol. Chem. 275: 8121–8125

  • Lee D, Lee B, Kim J, Kim DW, Choe J . 2000 J. Biol. Chem. 275: 7045–7051

  • Lee D, Hwang SG, Kim J, Choe J . 2002 J. Biol. Chem. 277: 6483–6489

  • Li R, Knight JD, Jackson SP, Tjian R, Botchan MR . 1991 Cell 65: 493–505

  • Masutani M, Nozaki T, Watanabe M, Ochiya T, Hasegawa F, Nakagama H, Suzuki H, Sugimura T . 2001 Mut. Res. 477: 111–117

  • McBride AA, Romanczuk H, Howley PM . 1991 J. Biol. Chem. 266: 18411–18414

  • Meisterernst M, Stelzer G, Roeder RG . 1997 Proc. Natl. Acad. Sci. USA 94: 2261–2265

  • Miyamoto T, Kakizawa T, Hashizume K . 1999 Mol. Cell. Biol. 19: 2644–2649

  • Molinete M, Vermeulen W, Burkle A, Menissier-de Murcia J, Kupper JH, Hoeijmakers JH, de Murcia G . 1993 EMBO J. 12: 2109–2117

  • Nie J, Sakamoto S, Song D, Qu Z, Ota K, Taniguchi T . 1998 FEBS Lett. 424: 27–32

  • Oei SL, Griesenbeck J, Schweiger M, Babich V, Kropotov A, Tomilin N . 1997 Biochem. Biophys. Res. Commun. 240: 108–111

  • Ohashi Y, Ueda K, Hayashi O, Ikai K, Niwa O . 1984 Proc. Natl. Acad. Sci. USA 81: 7132–7136

  • Ohashi Y, Itaya A, Tanaka Y, Yoshihara K, Kamiya T, Matsukage A . 1986 Biochem. Biophys. Res. Commun. 140: 666–673

  • Oliver FJ, Menissier-de Murcia J, Nacci C, Decker P, Andriantsitohaina R, Muller S, de la Rubia G, Stoclet JC, de Murcia G . 1999 EMBO J. 18: 4446–4454

  • Plaza S, Aumercier M, Bailly M, Dozier C, Saule S . 1999 Oncogene 18: 1041–1051

  • Pugh BF . 1995 Methods Mol. Biol. 37: 349–357

  • Rank NM, Lambert PF . 1995 J. Virol. 69: 6323–6334

  • Ruscetti T, Lehnert BE, Halbrook J, Le Trong H, Hoekstra MF, Chen DJ, Peterson SR . 1998 J. Biol. Chem. 273: 14461–14467

  • Sherman L, Schlegel R . 1996 J. Virol. 70: 3269–3279

  • Sherman L, Jackman A, Itzhaki H, Stoppler MC, Koval D, Schlegel R . 1997 Virology 237: 296–306

  • Simbulan-Rosenthal CM, Rosenthal DS, Luo R, Smulson ME . 1999 Oncogene 18: 5015–5023

  • Smulson ME, Kang VH, Ntambi JM, Rosenthal DS, Ding R, Simbulan CM . 1995 J. Biol. Chem. 270: 119–127

  • Steger G, Corbach S . 1997 J. Virol. 71: 50–58

  • Stubenrauch F, Lim HB, Laimins LA . 1998 J. Virol. 72: 1071–1077

  • Wang ZQ, Stingl L, Morrison C, Jantsch M, Los M, Schulze-Osthoff K, Wagner EF . 1997 Genes Dev. 11: 2347–2358

  • Wesierska-Gadek J, Schmid G, Cerni C . 1996 Biochem. Biophys. Res. Commun. 224: 96–102

  • Webster K, Parish J, Pandya M, Stern PL, Clarke AR, Gaston K . 2000 J. Biol. Chem. 275: 87–94

  • Yoshihara K, Itaya A, Tanaka Y, Ohashi Y, Ito K, Teraoka H, Tsukada K, Matsukage A, Kamiya T . 1985 Biochem. Biophys. Res. Commun. 128: 61–67

  • Zhang X, Morera S, Bates PA, Whitehead PC, Coffer AI, Hainbucher K, Nash RA, Sternberg MJ, Lindahl T, Freemont PS . 1998 EMBO J. 17: 6404–6411

  • zur Hausen H . 1996 Biochim. Biophys. Acta 1288: F55–F78

  • zur Hausen H . 2000 J. Natl. Cancer. Inst. 92: 690–698

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Acknowledgements

This work was supported in part by the National Research Laboratory Program of the Korea Institute of Science and Technology Evaluation and Planning (KISTEP), by the Molecular Medicine Research Group Program of KISTEP through the Biomedical Research Center at Korea Advanced Institute of Science and Technology, and by the BK21 Program of the Ministry of Education, Republic of Korea.

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Correspondence to Josiane Ménissier-de Murcia.

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Lee, D., Kim, J., Kim, K. et al. Functional interaction between human papillomavirus type 18 E2 and poly(ADP-ribose) polymerase 1. Oncogene 21, 5877–5885 (2002). https://doi.org/10.1038/sj.onc.1205723

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